Skip Navigation


JAC Advance Access first published online on October 18, 2008
This version published online on October 21, 2008
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkn428
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Data
Right arrow All Versions of this Article:
63/1/67    most recent
dkn428v2
dkn428v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Marcadé, G.
Right arrow Articles by Arlet, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marcadé, G.
Right arrow Articles by Arlet, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Replicon typing of plasmids in Escherichia coli producing extended-spectrum β-lactamases

Geraldine Marcadé1,2, Catherine Deschamps3, Anders Boyd4, Valérie Gautier1,2, Bertrand Picard3, Catherine Branger3,5, Erick Denamur3 and Guillaume Arlet1,2,*

1 Université Pierre et Marie Curie-Paris-6, Faculté de Médecine, Site Saint-Antoine, Laboratoire de Bactériologie, EA 2392 Paris, France 2 Assistance Publique-Hôpitaux de Paris, Hôpital Tenon, Service de Bactériologie-Hygiène, Paris, France 3 INSERM U722 and Université Denis Diderot-Paris-7, Faculté de Médecine, Site Xavier Bichat, Paris, France 4 INSERM U707, Université Pierre et Marie Curie-Paris-6, Faculté de Médecine, Site Saint-Antoine, Paris, France 5 Assistance Publique-Hôpitaux de Paris, Hôpital Louis Mourier, Laboratoire de Microbiologie, Colombes, France

Received 3 May 2008; returned 23 June 2008; revised 20 September 2008; accepted 22 September 2008

* Correspondence address. Laboratoire de Bactériologie, Hôpital Tenon, 4 rue de la Chine, 75970 Paris Cedex 20, France. Tel: +33-1-56-01-70-18; Fax: +33-1-56-01-61-08; E-mail: guillaume.arlet{at}tnn.aphp.fr

Objectives: Escherichia coli producing CTX-M-15 and CTX-M-14 extended-spectrum β-lactamases (ESBLs) are spreading worldwide. The aim of this work was to investigate the replicons involved in the emergence and spread of ESBLs in relation to ESBL type.

Methods: A collection of 125 TEM, SHV and CTX-M ESBL-producing E. coli strains was analysed. The replicons carrying the ESBLs and the total plasmid content of the strains have been characterized by PCR replicon typing in relation to the type of ESBL. The ESBL replicons were then compared with the replicon content of E. coli strains carrying TEM-1 or inhibitor-resistant TEM (IRT) β-lactamases.

Results: IncF plasmids were the most frequently carried replicons in our collection, but none carried TEM ESBL. Of TEM ESBLs, 67% were carried on IncA/C replicons except for TEM-52 genes, which were carried preferentially on IncI1 replicons. Although CTX-M enzymes can be carried by various replicons, the great majority of genes encoding CTX-M-14 and CTX-M-15 ESBLs were carried by IncF replicons, as were TEM-1 and IRT β-lactamases.

Conclusions: Resistance genes borne by the narrow host-range IncF replicon spread readily as this replicon is well adapted to E. coli. This is observed for blaTEM-1 and blaCTX-M-15 and, to a lesser extent, for blaCTX-M-14. Transposition immunity seems to play an important role in the diffusion process.

Key Words: PCR-based replicon typing , ESBLs , E. coli , transposition immunity

The original version of this paper was incorrect. In the penultimate paragraph of the text on page 4, it should read: Kluyvera spp. are the ancestral host of CTX-M enzymes, so we know that these resistance genes came from the environment.1 Some of these genes, for example, blaCTX-M-1 and blaCTX-M-3, were mobilized by ISEcp1 probably on non-IncF replicons (IncL/M, IncN and IncI1).1


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
V. Duval, I. Maiga, A. Maiga, T. Guillard, L. Brasme, D. Forte, J. Madoux, V. Vernet-Garnier, and C. De Champs
High Prevalence of CTX-M-Type {beta}-Lactamases among Clinical Isolates of Enterobacteriaceae in Bamako, Mali
Antimicrob. Agents Chemother., November 1, 2009; 53(11): 4957 - 4958.
[Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
J. Hrabak, J. Empel, T. Bergerova, K. Fajfrlik, P. Urbaskova, I. Kern-Zdanowicz, W. Hryniewicz, and M. Gniadkowski
International Clones of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum {beta}-Lactamases in a Czech Hospital
J. Clin. Microbiol., October 1, 2009; 47(10): 3353 - 3357.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
E. Ruppe, P.-L. Woerther, A. Diop, A.-M. Sene, A. Da Costa, G. Arlet, A. Andremont, and B. Rouveix
Carriage of CTX-M-15-Producing Escherichia coli Isolates among Children Living in a Remote Village in Senegal
Antimicrob. Agents Chemother., July 1, 2009; 53(7): 3135 - 3137.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. Carattoli
Resistance Plasmid Families in Enterobacteriaceae
Antimicrob. Agents Chemother., June 1, 2009; 53(6): 2227 - 2238.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.