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JAC Advance Access published online on September 23, 2008
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkn397
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© The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Complete nucleotide sequence of pKP96, a 67850 bp multiresistance plasmid encoding qnrA1, aac(6')-Ib-cr and blaCTX-M-24 from Klebsiella pneumoniae

Ping Shen1, Yan Jiang1,2, Zhihui Zhou1, Junli Zhang1, Yunsong Yu1,* and Lanjuan Li1

1 State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China 2 James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou, Zhejiang 310008, China

Received 19 May 2008; returned 3 July 2008; revised 23 August 2008; accepted 27 August 2008

* Corresponding author. Tel: +86-571-8723-6421; Fax: +86-571-8723-6423; E-mail: yvys119{at}163.com

Objectives: The multiresistance plasmid pKP96 from Klebsiella pneumoniae was sequenced completely and analysed concerning its genetic environment and distributing of antimicrobial resistance genes.

Methods: The complete sequence of the plasmid was determined using a whole-genome shotgun approach. MICs of 13 antimicrobial agents were determined using Etests. A conjugation experiment was performed in liquid medium.

Results: pKP96 is a circularly closed 67 850 bp multiresistance plasmid with an IncN incompatibility group. Seventy putative genes were identified according to the annotation of the finished sequence. The backbone region of the plasmid, comprising the conjugal transfer and plasmid replication regions, showed 91% identity to the IncN plasmid R46. Several mobile elements were found to be inserted into pKP96 together with antimicrobial resistance genes, including qnrA1, aac(6')-Ib-cr and blaCTX-M-24.

Conclusions: Plasmid pKP96 is a chimera that has acquired its multiple antimicrobial resistance determinants horizontally from different sources. It may have evolved from an ancestor plasmid similar to R46 through the stepwise events of integration or recombination.

Key Words: integrons , transposons , horizontal gene transfer


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