JAC Advance Access published online on April 1, 2008
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkn145
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Original research |
Dissemination of ESBL and Qnr determinants in Enterobacter cloacae in Algeria
1 Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene, BP 32 El-Alia, Bab-Ezzouar 16111, Alger, Algérie 2 Laboratoire de Bactériologie, CHU Beni Messous, Alger, Algérie 3 Service de Microbiologie, CHU Mustapha Bacha, Alger, Algérie 4 Université Paris VI, Faculté de Médecine Pierre et Marie Curie, Laboratoire de Bactériologie, UPRES EA 2392, Paris, France
Received 18 January 2008; returned 31 January 2008; revised 10 March 2008; accepted 10 March 2008
* Correspondence address. Service de Bactériologie-Hygiène, Hôpital Tenon, 4 rue de la Chine, 75970 Paris cedex 20, France. Tel: +33-1-56-01-70-18; Fax: +33-1-56-01-61-08; E-mail: guillaume.arlet{at}tnn.aphp.fr
Objectives: The aim of this study is to evaluate the prevalence and diversity of extended-spectrum β-lactamases (ESBLs) in Enterobacter cloacae clinical isolates collected from Algerian hospitals and to verify the association with qnr genes.
Methods: MICs were determined by Etest for isolates giving positive double-disc synergy tests, and all isolates were screened by PCR and sequenced, respectively, for blaTEM, blaCTX-M, blaSHV and blaVEB genes and for qnr genes (qnrA, qnrB, qnrS), using specific primers.
Results: The prevalence of ESBLs was 25/141 (17.7%) with 11, 9, 4 and 1 isolates testing positive for genes encoding CTX-M-15, CTX-M-3, SHV-12 and VEB-1, respectively. Two SHV-12 producers and one CTX-M-15 producer expressed QnrS1, one isolate produced CTX-M-15 and QnrB1 and one SHV-12 producer co-expressed QnrS1 and QnrB4. qnrA was not detected in our collection, and qnr alleles were not detected in non-ESBL-producing isolates.
Conclusions: SHV-12, QnrS1, QnrB1 and QnrB4 were reported for the first time in Algeria. This study also described a co-expression of qnrS1 and qnrB4 by an SHV-12 producer isolate.
Key Words: E. cloacae , CTX-M , SHV-12 , QnrB , QnrS
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. T. Minh Vien, S. Baker, L. T. Phuong Thao, L. T. Phuong Tu, C. Thu Thuy, T. T. Thu Nga, N. V. Minh Hoang, J. I. Campbell, L. Minh Yen, N. Trong Hieu, et al. High prevalence of plasmid-mediated quinolone resistance determinants in commensal members of the Enterobacteriaceae in Ho Chi Minh City, Vietnam J. Med. Microbiol., December 1, 2009; 58(12): 1585 - 1592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Strahilevitz, G. A. Jacoby, D. C. Hooper, and A. Robicsek Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat Clin. Microbiol. Rev., October 1, 2009; 22(4): 664 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Iabadene, C. Dallenne, Y. Messai, D. Geneste, R. Bakour, and G. Arlet Emergence of Extended-Spectrum {beta}-Lactamase PER-1 in Proteus vulgaris and Providencia stuartii Isolates from Algiers, Algeria Antimicrob. Agents Chemother., September 1, 2009; 53(9): 4043 - 4044. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Garcia-Fernandez, D. Fortini, K. Veldman, D. Mevius, and A. Carattoli Characterization of plasmids harbouring qnrS1, qnrB2 and qnrB19 genes in Salmonella J. Antimicrob. Chemother., February 1, 2009; 63(2): 274 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pomba, J. D. da Fonseca, B. C. Baptista, J. D. Correia, and L. Martinez-Martinez Detection of the Pandemic O25-ST131 Human Virulent Escherichia coli CTX-M-15-Producing Clone Harboring the qnrB2 and aac(6')-Ib-cr Genes in a Dog Antimicrob. Agents Chemother., January 1, 2009; 53(1): 327 - 328. [Full Text] [PDF]
|
|