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JAC Advance Access published online on March 20, 2008
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkn122
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© The Author 2008. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Original research

Microbiological evaluation of a new growth-based approach for rapid detection of methicillin-resistant Staphylococcus aureus

Christof von Eiff1,*, Dominik Maas1, Gunnar Sander1, Alexander W. Friedrich2, Georg Peters1 and Karsten Becker1

1 Institute of Medical Microbiology, University Hospital of Münster, Domagkstr. 10, 48149 Münster, Germany 2 Institute for Hygiene, University Hospital of Münster, Robert-Koch-Str. 41, 48149 Münster, Germany

Received 18 January 2008; returned 12 February 2008; revised 26 February 2008; accepted 26 February 2008

* Corresponding author. Tel: +49-251-83-55360; Fax: +49-251-83-55350; E-mail: eiffc{at}uni-muenster.de

Objectives: Recently, a rapid screening tool for methicillin-resistant Staphylococcus aureus (MRSA) has been introduced that applies a novel detection technology allowing the rapid presence or absence of MRSA to be determined from an enrichment broth after only a few hours of incubation. To evaluate the reliability of this new assay to successfully detect MRSA strains of different origin and clonality, well-characterized S. aureus strains were tested in this study.

Methods: More than 700 methicillin-susceptible and methicillin-resistant strains covering >90% of all registered European MRSA spa types within the SeqNet network were studied.

Results: All 513 MRSA strains tested were recognized as methicillin-resistant: among these, 96 MRSA strains were from an institutional collection, each presenting a unique spa type. None of the 211 methicillin-susceptible strains were detected as positive.

Conclusions: The new growth-based rapid MRSA assay was shown to detect without exception all MRSA strains of large collections of strains comprising highly diverse genetic backgrounds, indicating that such a phenotypic test might be potentially more likely to cope with new strains.

Key Words: staphylococci , MRSA , infection control , spa typing


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