JAC Advance Access published online on February 21, 2008
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkn068
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Original research |
Surveillance for plasmid-mediated quinolone resistance determinants in Enterobacteriaceae within the Calgary Health Region, Canada: the emergence of aac(6')-Ib-cr
1 Division of Microbiology, Calgary Laboratory Services, University of Calgary, #9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8; 2 Department of Pathology and Laboratory Medicine, University of Calgary, #9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8; 3 Department of Microbiology and Infectious Diseases, University of Calgary, #9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8; 4 Department of Medicine, University of Calgary, #9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8
Received 19 December 2007; returned 25 January 2008; revised 22 January 2008; accepted 28 January 2008
* Corresponding author. Division of Microbiology, Calgary Laboratory Services, #9, 3535 Research Road NW, Calgary, Alberta, Canada T2L 2K8. Tel: +1-403-770-3309; Fax: +1-403-770-3347; E-mail: johann.pitout{at}cls.ab.ca
Objectives: A study was designed to determine the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants among clinical isolates of Enterobacteriaceae from the Calgary Health Region (CHR).
Methods: During January and February 2004 and January and February 2007, 564 non-repeat isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis and Morganella morganii resistant to ciprofloxacin and/or tobramycin were screened for PMQR determinants using a multiplex PCR for qnrA, qnrS and qnrB and aac(6')-Ib genes; aac(6')-Ib-cr was further identified by digestion with BstF5I.
Results: In 2004, 6/139 (4%) of the resistant E. coli were positive for aac(6')-Ib-cr. In 2007, 53/398 (13%) were positive for aac(6')-Ib-cr, 3/398 (0.8%) were positive for qnrS, and one isolate was positive for both. All the isolates were present in urines and the majority [40/63 (63%)] were submitted from community collection sites; 8 (13%) isolates co-produced AmpC β-lactamases and 34 (54%) co-produced CTX-M-15. aac(6')-Ib-cr was present in one Klebsiella pneumoniae and one P. mirabilis, whereas one isolate of K. pneumoniae was positive for both aac(6')-Ib-cr and qnrB.
Conclusions: Our results showed that isolates with aac(6')-Ib-cr, often associated with CTX-M-15, are emerging among fluoroquinolone-resistant E. coli in the CHR. Our study suggests that surveillance for PMQR determinants should be undertaken on a regular basis.
Key Words: PMQR , CTX-M-15 , Escherichia coli
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Strahilevitz, G. A. Jacoby, D. C. Hooper, and A. Robicsek Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat Clin. Microbiol. Rev., October 1, 2009; 22(4): 664 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. D. Pitout, D. B. Gregson, L. Campbell, and K. B. Laupland Molecular Characteristics of Extended-Spectrum-{beta}-Lactamase-Producing Escherichia coli Isolates Causing Bacteremia in the Calgary Health Region from 2000 to 2007: Emergence of Clone ST131 as a Cause of Community-Acquired Infections Antimicrob. Agents Chemother., July 1, 2009; 53(7): 2846 - 2851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Carattoli Resistance Plasmid Families in Enterobacteriaceae Antimicrob. Agents Chemother., June 1, 2009; 53(6): 2227 - 2238. [Full Text] [PDF]
|
|
|
|
|
|
J. D. D. Pitout, L. Campbell, D. L. Church, D. B. Gregson, and K. B. Laupland Molecular Characteristics of Travel-Related Extended-Spectrum-{beta}-Lactamase-Producing Escherichia coli Isolates from the Calgary Health Region Antimicrob. Agents Chemother., June 1, 2009; 53(6): 2539 - 2543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sjolund-Karlsson, J. P. Folster, G. Pecic, K. Joyce, F. Medalla, R. Rickert, and J. M. Whichard Emergence of Plasmid-Mediated Quinolone Resistance among Non-Typhi Salmonella enterica Isolates from Humans in the United States Antimicrob. Agents Chemother., May 1, 2009; 53(5): 2142 - 2144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Jacoby, N. Gacharna, T. A. Black, G. H. Miller, and D. C. Hooper Temporal Appearance of Plasmid-Mediated Quinolone Resistance Genes Antimicrob. Agents Chemother., April 1, 2009; 53(4): 1665 - 1666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Warburg, M. Korem, A. Robicsek, D. Engelstein, A. E. Moses, C. Block, and J. Strahilevitz Changes in aac(6')-Ib-cr Prevalence and Fluoroquinolone Resistance in Nosocomial Isolates of Escherichia coli Collected from 1991 through 2005 Antimicrob. Agents Chemother., March 1, 2009; 53(3): 1268 - 1270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cui, J. Li, Z. Sun, C. Hu, S. Jin, F. Li, Y. Guo, L. Ran, and Y. Ma Characterization of Salmonella enterica isolates from infants and toddlers in Wuhan, China J. Antimicrob. Chemother., January 1, 2009; 63(1): 87 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ll. Jones, R. E. Warren, S. J. Skidmore, V. A. Davies, T. Gibreel, and M. Upton Prevalence and distribution of plasmid-mediated quinolone resistance genes in clinical isolates of Escherichia coli lacking extended-spectrum {beta}-lactamases J. Antimicrob. Chemother., December 1, 2008; 62(6): 1245 - 1251. [Abstract] [Full Text] [PDF]
|
|