JAC Advance Access published online on November 20, 2007
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkm448
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Prevalence of qnr genes among extended-spectrumß-lactamase-producing enterobacterial isolatesin Barcelona, Spain
1 Department of Microbiology, Hospital Vall d’Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain 2 Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
Received 1 August 2007; returned 18 August 2007; revised 22 October 2007; accepted 22 October 2007
* Corresponding author. Tel: +34-93-2746817; Fax: +34-93-2746801; E-mail: gprats{at}vhebron.net
Objectives: To evaluate the presence of qnr genes among enterobacterial isolates carrying extended-spectrum ß-lactamases (ESBLs) in Barcelona, Spain.
Methods: Screening for the qnrA, qnrB and qnrS genes was carried out by PCR amplification with specific primers in 305 non-duplicate, clinically relevant ESBL-producing enterobacterial isolates obtained from February 2003 to August 2004. ESBLs from all qnr-positive isolates were characterized by isoelectric focusing, PCR amplification and DNA sequencing. Plasmid analysis was performed by S1 digestion and hybridization with specific probes for the qnr and bla genes. Plasmids containing qnr genes were transferred by conjugation or transformation. The genetic environment of qnrA1 in selected isolates was characterized by cloning experiments.
Results: Fifteen isolates, each from a different individual, carried qnr. Among them, 14 had qnrA1 (6 Klebsiella pneumoniae, 6 Enterobacter cloacae and 2 Escherichia coli isolates) and 1 had qnrS1 (K. pneumoniae). None of the isolates carried qnrB. Among the qnrA1-carrying isolates, 10 possessed both blaCTX-M-9 and blaSHV-12, 2 had both blaCTX-M-9 and blaSHV-92 and 2 had blaCTX-M-9 alone. The isolate with qnrS1 possessed blaSHV-12. The qnrA1 and ESBL genes were located together on plasmids ranging in size from 40 to 320 kb. qnrS1 and blaSHV-12 were not located on the same plasmid. Transfer of quinolone resistance was successfully achieved from all but three isolates. The cloned region surrounding qnrA in two K. pneumoniae isolates revealed a novel genetic organization.
Conclusions: The prevalence of qnr among enterobacterial clinical isolates carrying ESBLs between 2003 and 2004 in Barcelona was 4.9%. qnrA1 was the most prevalent, whereas only one qnrS and no qnrB were detected.
Key Words: ESBLs , quinolones , enterobacteria
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Strahilevitz, G. A. Jacoby, D. C. Hooper, and A. Robicsek Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat Clin. Microbiol. Rev., October 1, 2009; 22(4): 664 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Allou, E. Cambau, L. Massias, F. Chau, and B. Fantin Impact of Low-Level Resistance to Fluoroquinolones Due to qnrA1 and qnrS1 Genes or a gyrA Mutation on Ciprofloxacin Bactericidal Activity in a Murine Model of Escherichia coli Urinary Tract Infection Antimicrob. Agents Chemother., October 1, 2009; 53(10): 4292 - 4297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Hawkey and A. M. Jones The changing epidemiology of resistance J. Antimicrob. Chemother., September 1, 2009; 64(suppl_1): i3 - i10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Gonzalez-Lopez, A. Coelho, M. N. Larrosa, S. Lavilla, R. Bartolome, and G. Prats First Detection of Plasmid-Encoded blaOXY {beta}-Lactamase Antimicrob. Agents Chemother., July 1, 2009; 53(7): 3143 - 3146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sanchez-Cespedes, M. D. Blasco, S. Marti, V. Alba, E. Alcalde, C. Esteve, and J. Vila Plasmid-Mediated QnrS2 Determinant from a Clinical Aeromonas veronii Isolate Antimicrob. Agents Chemother., August 1, 2008; 52(8): 2990 - 2991. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cagnacci, L. Gualco, E. Debbia, G. C. Schito, and A. Marchese European Emergence of Ciprofloxacin-Resistant Escherichia coli Clonal Groups O25:H4-ST 131 and O15:K52:H1 Causing Community-Acquired Uncomplicated Cystitis J. Clin. Microbiol., August 1, 2008; 46(8): 2605 - 2612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lascols, I. Podglajen, C. Verdet, V. Gautier, L. Gutmann, C.-J. Soussy, E. Collatz, and E. Cambau A Plasmid-Borne Shewanella algae Gene, qnrA3, and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae J. Bacteriol., August 1, 2008; 190(15): 5217 - 5223. [Abstract] [Full Text] [PDF]
|
|