JAC Advance Access published online on February 8, 2007
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl535
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Genetic relatedness between methicillin-susceptible and methicillin-resistant Staphylococcus aureus: results of a national survey
Laboratoire de Référence MRSA-Staphylocoques, Service de Microbiologie, Université Libre de Bruxelles, Hôpital Erasme, 1070 Brussels, Belgium
Received 3 October 2006; returned 26 October 2006; revised 11 December 2006; accepted 11 December 2006
* Corresponding author. Tel: +32-2-555-34-84; Fax: +32-2-555-31-10; E-mail: mahallin{at}ulb.ac.be
OBJECTIVES: Surveillance of hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA) infections has shown the emergence and spread of several epidemic MRSA clones over the past 10 years in Belgium. Whether these clones have been imported from abroad or else have arisen locally via staphylococcal cassette chromosome mec (SCCmec) acquisition by successful methicillin-susceptible S. aureus (MSSA) clones is unknown.
METHODS: We determined by PFGE, spa typing, multi-locus sequence typing (MLST) and agr group analysis the genetic relatedness of 103 MSSA and 511 MRSA strains from a national survey of patients admitted to 112 Belgian hospitals in 2003.
RESULTS: The 103 MSSA strains presented very diverse genetic backgrounds, they were distributed into 40 distinct PFGE types and clustered in 15 distinct MLST CCs. Up to 45% harboured the same genotype as five major epidemic HA-MRSA clones. These MRSA clones all harbour a type IV SCCmec element.
CONCLUSIONS: These findings are consistent with multiple recent acquisitions of the more mobile type IV SCCmec by MSSA and suggest that certain genetic backgrounds are conferring a selective advantage, regardless of the resistance profile. However, since the predominant MSSA and MRSA lineages identified in Belgium are disseminated worldwide, importation of epidemic MRSA strains remains an alternative hypothesis.
Key Words: epidemiology , evolution , pulsed field gel electrophoresis , Belgium
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  O. Denis, B. Jans, A. Deplano, C. Nonhoff, R. De Ryck, C. Suetens, and M. J. Struelens Epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) among residents of nursing homes in Belgium J. Antimicrob. Chemother., December 1, 2009; 64(6): 1299 - 1306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Argudin, M. C. Mendoza, F. J. Mendez, M. C. Martin, B. Guerra, and M. R. Rodicio Clonal Complexes and Diversity of Exotoxin Gene Profiles in Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Patients in a Spanish Hospital J. Clin. Microbiol., July 1, 2009; 47(7): 2097 - 2105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. El Garch, M. Hallin, R. De Mendonca, O. Denis, A. Lefort, and M. J. Struelens StaphVar-DNA microarray analysis of accessory genome elements of community-acquired methicillin-resistant Staphylococcus aureus J. Antimicrob. Chemother., May 1, 2009; 63(5): 877 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Nguyen, O. Denis, A. Vergison, A. Theunis, P. M. Tulkens, M. J. Struelens, and F. Van Bambeke Intracellular Activity of Antibiotics in a Model of Human THP-1 Macrophages Infected by a Staphylococcus aureus Small-Colony Variant Strain Isolated from a Cystic Fibrosis Patient: Pharmacodynamic Evaluation and Comparison with Isogenic Normal-Phenotype and Revertant Strains Antimicrob. Agents Chemother., April 1, 2009; 53(4): 1434 - 1442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Perez-Vazquez, A. Vindel, C. Marcos, J. Oteo, O. Cuevas, P. Trincado, V. Bautista, H. Grundmann, J. Campos, and on behalf of the EARSS Spain spa-typing Group Spread of invasive Spanish Staphylococcus aureus spa-type t067 associated with a high prevalence of the aminoglycoside-modifying enzyme gene ant(4')-Ia and the efflux pump genes msrA/msrB J. Antimicrob. Chemother., January 1, 2009; 63(1): 21 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Nubel, P. Roumagnac, M. Feldkamp, J.-H. Song, K. S. Ko, Y.-C. Huang, G. Coombs, M. Ip, H. Westh, R. Skov, et al. Frequent emergence and limited geographic dispersal of methicillin-resistant Staphylococcus aureus PNAS, September 16, 2008; 105(37): 14130 - 14135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-L. Hu, K. Omoe, F. Inoue, T. Kasai, M. Yasujima, K. Shinagawa, and A. Nakane Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates J. Med. Microbiol., September 1, 2008; 57(9): 1106 - 1112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Roisin, C. Nonhoff, O. Denis, and M. J. Struelens Evaluation of New Vitek 2 Card and Disk Diffusion Method for Determining Susceptibility of Staphylococcus aureus to Oxacillin J. Clin. Microbiol., August 1, 2008; 46(8): 2525 - 2528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nulens, E. E. Stobberingh, H. van Dessel, S. Sebastian, F. H. van Tiel, P. S. Beisser, and R. H. Deurenberg Molecular Characterization of Staphylococcus aureus Bloodstream Isolates Collected in a Dutch University Hospital between 1999 and 2006 J. Clin. Microbiol., July 1, 2008; 46(7): 2438 - 2441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Strommenger, C. Braulke, D. Heuck, C. Schmidt, B. Pasemann, U. Nubel, and W. Witte spa Typing of Staphylococcus aureus as a Frontline Tool in Epidemiological Typing J. Clin. Microbiol., February 1, 2008; 46(2): 574 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Witte, B. Strommenger, C. Cuny, D. Heuck, and U. Nuebel Methicillin-resistant Staphylococcus aureus containing the Panton-Valentine leucocidin gene in Germany in 2005 and 2006 J. Antimicrob. Chemother., December 1, 2007; 60(6): 1258 - 1263. [Abstract] [Full Text] [PDF]
|
|