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JAC Advance Access published online on October 23, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl418
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received June 22, 2006
Revised September 18, 2006
Accepted September 18, 2006

Brief report

Fusidic acid resistance, mediated by fusB, in bovine coagulase-negative staphylococci

Siamak P. Yazdankhah 1 *, Anette W. Åsli 1, Henning Sørum 1, Hanne Oppegaard 2, and Marianne Sunde 3

1 Department of Food Safety and Infection Biology, The Norwegian School of Veterinary Science, Oslo, Norway
2 TINE Norwegian Dairies BA, R&D Centre, Oslo, Norway
3 Section of Bacteriology, National Veterinary Institute, Oslo, Norway

* To whom correspondence should be addressed.
Siamak P. Yazdankhah, E-mail: siamak.yazdankhah{at}fhi.no


  Abstract

Objectives: The aim of this study was to determine the occurrence of fusidic acid resistance, mediated by the fusB gene, among coagulase-negative staphylococci (CoNS) isolated from bovine mastitis.

Methods: A total of 113 CoNS isolates were screened for susceptibility to fusidic acid by using a disc diffusion method. The fusB gene was detected by using PCR and subsequent DNA sequencing. The localization of fusB was determined by hybridization.

Results: The fusB gene was detected in 3 of 11 fusidic acid-resistant bovine CoNS isolates. The organization of the fusB downstream region on a 40 kb plasmid in a Staphylococcus haemolyticus isolate (288/96) was highly similar to the previously reported organization of fusB on plasmid pUB101 from a Staphylococcus aureus isolate of human origin. The fusB gene was chromosomally located in the remaining two isolates.

Conclusions: Fusidic acid resistance mediated by fusB is not the dominant resistance mechanism in fusidic acid-resistant CoNS studied in this work. The similarity between the organization of the fusB downstream region in S. haemolyticus (isolate 288/96) and on plasmid pUB101 from an S. aureus isolate of human origin indicates a common ancestral origin of these genes.

Keywords: bovine mastitis; plasmid; S. haemolyticus.
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