JAC Advance Access published online on October 20, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl400
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1 Departamento de Biología Funcional-Microbiología, Universidad de Oviedo and Instituto Universitario de Biotecnología de Asturias (IUBA), 33006-Oviedo, Spain
* To whom correspondence should be addressed. Objectives: To characterize class 1 and class 2 integrons which were simultaneously detected in non-typhoid Salmonella enterica strains of non-prevalent serovars, and to investigate their possible association with transposons and/or plasmids. Methods: Eight multidrug-resistant S. enterica strains belonging to serovars Virchow (4), Panama (2), Grumpensis (1) and Worthington (1), each containing a class 1 and a class 2 integron, were analysed. Nested PCR amplification was used to determine the gene-cassette configuration of the integrons. Overlapping PCR amplifications were applied in integron-transposon linkage experiments. Conjugation and hybridization experiments were used to localize integrons and transposons in the bacterial genome (plasmid and chromosome associated). Results: One of two different class 1 integrons (with variable regions of 1000 bp/aadA1 and 2300 bp/sat-smr-aadA1) inserted into Tn21-like transposons, were found to coexist with the class 2 integron (2300 bp/dfrA1-sat1-aadA1) of Tn7 in the analysed strains. Class 1 integrons were always found in large conjugative plasmids whereas apparently intact or defective copies of the Tn7 integron could be located on the same plasmid and/or the bacterial chromosome. Conclusions: This report describes different associations between mobile genetic elements that play a crucial role in the capture and spread of antimicrobial drug resistance. As far as we are aware, this is the first description of class 2 integrons in serovars Panama, Grumpensis and Worthington.
Received July 26, 2006
Revised September 7, 2006
Accepted September 11, 2006
Original article
Class 1 and class 2 integrons in non-prevalent serovars of Salmonella enterica: structure and association with transposons and plasmids
Irene Rodríguez 1, M. Cruz Martín 2, M. Carmen Mendoza 1, and M. Rosario Rodicio 1 *
2 Instituto de Productos Lácteos de Asturias (CSIC), 33300-Villaviciosa, Asturias, Spain
M. Rosario Rodicio, E-mail: rrodicio{at}fq.uniovi.es
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