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JAC Advance Access published online on September 26, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl397
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© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received May 31, 2006
Revised August 25, 2006
Accepted September 11, 2006

Brief report

Emergence of CTX-M-12 extended-spectrum {beta}-lactamase-producing Escherichia coli in Korea

Il Kwon Bae 1, You-Nae Lee 1, Hyun Yong Hwang 1, Seok Hoon Jeong 1 *, Su Jin Lee 2, Hyo-Sun Kwak 3, Wonkeun Song 4, Hyoung Jin Kim 5, and Hasik Youn 5

1 Department of Laboratory Medicine, Kosin University College of Medicine, 602-030, 34 Amnam-Dong, Suh-Gu, Busan, Korea
2 Department of Quality Improvement, Pusan National University Hospital, 602-739, 1-10 Ami-Dong, Suh-Gu, Busan, Korea
3 Center for Food Safety Evaluation, Korea Food and Drug Administration, 122-704, 231 Jinheung-Ro, Eunpyung-Gu, Seoul, Korea
4 Department of Laboratory Medicine, Hallym University College of Medicine, 150-950, 948-1 Daerim 1-Dong, Yongdeungpo-Gu, Seoul, Korea
5 R&D Park, LG Life Sciences, Ltd, 305-380, 104-1 Moonji-Dong, Yuseong-Gu, Daejeon, Korea

* To whom correspondence should be addressed.
Seok Hoon Jeong, E-mail: kscpjsh{at}ns.kosinmed.or.kr


  Abstract

Objectives: To characterize CTX-M-12 extended-spectrum {beta}-lactamase (ESBL) produced by clinical Escherichia coli isolates and to investigate its genetic environment.

Methods: Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the double-disc synergy test was carried out. Detection of genes encoding class A {beta}-lactamases was performed by PCR amplification, and the genetic environments of the blaCTX-M-12 genes were investigated by PCR and sequencing of the regions surrounding the genes. Kinetic parameters were determined from purified CTX-M-12.

Results: Sequence data for the CTX-M-1 cluster from three clinical E. coli isolates indicated the presence of CTX-M-12. An ISEcp1 insertion sequence was located 49 bp upstream of blaCTX-M-12 in all three E. coli isolates. CTX-M-12 had a more potent hydrolytic activity against cefotaxime than against ceftazidime and was encoded on a self-transferable ~18 kbp plasmid.

Conclusions: This work shows that CTX-M-12, which confers high-level resistance to cefotaxime but not to ceftazidime, has emerged in Korea. The blaCTX-M-12 gene was associated with an upstream ISEcp1 insertion sequence.

Keywords: ISEcp1; horizontal transfer; ERIC-PCR.
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