Skip Navigation



JAC Advance Access published online on September 29, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl395
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
58/6/1295    most recent
dkl395v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Mariné, M.
Right arrow Articles by Guarro, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mariné, M.
Right arrow Articles by Guarro, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received June 28, 2006
Revised August 24, 2006
Accepted September 9, 2006

Brief report

Combined antifungal therapy in a murine infection by Candida glabrata

Marçal Mariné 1, Carolina Serena 1, F. Javier Pastor 1 *, and Josep Guarro 1

1 Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Carrer Sant Llorenç 21, 43201, Reus, Spain

* To whom correspondence should be addressed.
F. Javier Pastor, E-mail: franciscojavier.pastor{at}urv.cat


  Abstract

Objectives: To develop proper treatments for patients who do not respond to current antifungal treatments, we tested new combinations of antifungal drugs for treating disseminated infections by Candida glabrata in a murine model.

Methods: Mice were rendered neutropenic by intraperitoneal cyclophosphamide and intravenous 5-fluorouracil administration. The animals were infected intravenously with 2 x 108 cfu of C. glabrata. The efficacies of micafungin combined with amphotericin B, fluconazole or flucytosine, and of amphotericin B combined with fluconazole were evaluated by survival and tissue burden reduction.

Results and Conclusions: Micafungin plus amphotericin B was the most effective combination at reducing tissue burden. Micafungin at 10 mg/kg combined with amphotericin B at 0.75, 1.5 or 3 mg/kg prolonged survival with respect to the monotherapies, but only the second combination showed a synergistic effect in reducing fungal load in spleen and kidney. Amphotericin B at 1.5 mg/kg combined with micafungin at 5, 10 or 20 mg/kg reduced tissue burden with respect to the monotherapies, but the effects of the three combinations were very similar. These results suggest that amphotericin B in combination with micafungin is promising for the treatment of disseminated C. glabrata infections.

Keywords: candidiasis; animal models; micafungin; fluconazole; flucytosine.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
N. Kiraz, I. Dag, M. Yamac, A. Kiremitci, N. Kasifoglu, and Y. Akgun
Antifungal Activity of Caspofungin in Combination with Amphotericin B against Candida glabrata: Comparison of Disk Diffusion, Etest, and Time-Kill Methods
Antimicrob. Agents Chemother., February 1, 2009; 53(2): 788 - 790.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
C. Serena, M. M. Rodriguez, M. Marine, F. J. Pastor, and J. Guarro
Micafungin combined with fluconazole, an effective therapy for murine blastoschizomycosis
J. Antimicrob. Chemother., April 1, 2008; 61(4): 877 - 879.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
C. Serena, M. Marine, G. Quindos, A. J. Carrillo, J. F. Cano, F. J. Pastor, and J. Guarro
In Vitro Interactions of Micafungin with Amphotericin B against Clinical Isolates of Candida spp.
Antimicrob. Agents Chemother., April 1, 2008; 52(4): 1529 - 1532.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
M. M. Rodriguez, M. Ruiz, F. J. Pastor, G. Quindos, A. Carrillo, and J. Guarro
In vitro interaction of micafungin and fluconazole against Candida
J. Antimicrob. Chemother., July 1, 2007; 60(1): 188 - 190.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.