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JAC Advance Access published online on September 15, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl366
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received May 28, 2006
Revised August 14, 2006
Accepted August 14, 2006

Brief report

dfrA25, a novel trimethoprim resistance gene from Salmonella Agona isolated from a human urine sample in Brazil

Yvonne Agersø 1 *, Gisele Peirano 2, and Frank M. Aarestrup 1

1 WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens, Danish Institute for Food and Veterinary Research, Bulowsvej 27, DK-1790 Copenhagen V, Denmark
2 Oswaldo Cruz Institute, Avenida Brasil 4365, 21045-900 Rio de Janeiro, Brazil

* To whom correspondence should be addressed.
Yvonne Agersø, E-mail: ya{at}dfvf.dk


  Abstract

Objectives: To describe a novel trimethoprim resistance gene, designated dfrA25, which was detected as a gene cassette within a class 1 integron in Salmonella Agona.

Methods: The gene was cloned into Escherichia coli MT102 and resistance to 10 different antimicrobial drugs was measured. A phylogenetic tree was constructed based on representative trimethoprim-resistance-mediating DfrA proteins retrieved from GenBank. Filter-mating experiments and Southern blots of plasmid preparations were performed with the donor and selected transconjugants.

Results and conclusions: dfrA25 encodes a dihydrofolate reductase of 157 amino acids with closest identity (85%) to dfrA5 dihydrofolate reductase. dfrA25 was located on a transferable plasmid (~150 kb) that also harboured the tetracycline resistance gene tet(A).

Keywords: integrons; gene cassettes; dihydrofolate reductase.
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