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JAC Advance Access published online on April 26, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl150
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received January 25, 2006
Revised March 28, 2006
Accepted March 30, 2006

Brief report

A multiplex PCR for {beta}-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant

Stephen G. Tristram 1 * and Scott Nichols 1

1 School of Human Life Sciences, University of Tasmania, Launceston, Tasmania 7250, Australia

* To whom correspondence should be addressed.
Stephen G. Tristram, E-mail: Stephen.Tristram{at}utas.edu.au


  Abstract

Objectives: To establish a multiplex PCR to detect and differentiate the {beta}-lactamase genes of Haemophilus influenzae.

Methods: A collection of H. influenzae isolates with a range of different {beta}-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study.

Results: The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate blaROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region.

Conclusions: The multiplex PCR will be a useful tool for the surveillance of {beta}-lactamase genes in H. influenzae.

Keywords: H. influenzae; bla genes; TEM; ROB-1.
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