Skip Navigation



JAC Advance Access published online on March 23, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl098
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/6/1110    most recent
dkl098v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lee, J.-Y.
Right arrow Articles by Song, J.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, J.-Y.
Right arrow Articles by Song, J.-H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received January 8, 2006
Revised March 2, 2006
Accepted March 2, 2006

Original article

In vitro evaluation of the antibiotic lock technique (ALT) for the treatment of catheter-related infections caused by staphylococci

Ji-Young Lee 1 {dagger}, Kwan Soo Ko 1 {dagger}, Kyong Ran Peck 2 *, Won Sup Oh 2, and Jae-Hoon Song 3

1 Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Korea
2 Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Il-won dong, Kangnam-ku, Seoul 135-710, Korea
3 Asian-Pacific Research Foundation for Infectious Diseases (ARFID), Seoul, Korea; Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Il-won dong, Kangnam-ku, Seoul 135-710, Korea

* To whom correspondence should be addressed.
Kyong Ran Peck, E-mail: krpeck{at}smc.samsung.co.kr


  Abstract

Objectives: To investigate appropriate antimicrobial agents, the optimal concentration and treatment duration for the antibiotic lock technique (ALT) to treat catheter-related infections caused by Staphylococcus epidermidis and Staphylococcus aureus.

Methods: We evaluated the bacterial killing activity of vancomycin, teicoplanin, ciprofloxacin, rifampicin, cefazolin, gentamicin, nafcillin and erythromycin against biofilms formed by two strains of S. aureus and two strains of S. epidermidis. The effectiveness of the antibiotic lock was assayed after exposure to antibiotics (1, 5 and 10 mg/mL) for 1, 3, 5, 7, 10 or 14 days using an in vitro model of biofilms on polyurethane film.

Results: The biofilms were completely sterile after exposure to vancomycin (5 mg/mL) for 5 days and teicoplanin (5 and 10 mg/mL) for 7 days. Ciprofloxacin and rifampicin (both 5 mg/mL) achieved eradication of the biofilms of both staphylococcal species more rapidly than vancomycin or teicoplanin. Significant biofilm eradication was not achieved with cefazolin, nafcillin, gentamicin and erythromycin at any of the time exposures examined.

Conclusions: The data suggest that 5 mg/mL vancomycin, ciprofloxacin or rifampicin can eradicate S. epidermidis and S. aureus biofilms within 5 days. These findings warrant prospective clinical trials for the evaluation of the clinical efficacy of ALT for less than 10 days.

Keywords: Staphylococcus epidermidis; Staphylococcus aureus; biofilms; ciprofloxacin; rifampicin.
{dagger}These authors contributed equally to this work.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
Y. Qu, T. S. Istivan, A. J. Daley, D. A. Rouch, and M. A. Deighton
Comparison of various antimicrobial agents as catheter lock solutions: preference for ethanol in eradication of coagulase-negative staphylococcal biofilms
J. Med. Microbiol., April 1, 2009; 58(4): 442 - 450.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
W. E. Rose and P. T. Poppens
Impact of biofilm on the in vitro activity of vancomycin alone and in combination with tigecycline and rifampicin against Staphylococcus aureus
J. Antimicrob. Chemother., March 1, 2009; 63(3): 485 - 488.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
M. Y. Lee, K. S. Ko, J.-H. Song, and K. R. Peck
In vitro effectiveness of the antibiotic lock technique (ALT) for the treatment of catheter-related infections by Pseudomonas aeruginosa and Klebsiella pneumoniae
J. Antimicrob. Chemother., October 1, 2007; 60(4): 782 - 787.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
K. L. LaPlante and L. A. Mermel
In vitro activity of daptomycin and vancomycin lock solutions on staphylococcal biofilms in a central venous catheter model
Nephrol. Dial. Transplant., August 1, 2007; 22(8): 2239 - 2246.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.