Skip Navigation



JAC Advance Access published online on March 20, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl096
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Data
Right arrow All Versions of this Article:
57/6/1227    most recent
dkl096v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Hope, C. K.
Right arrow Articles by Wilson, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hope, C. K.
Right arrow Articles by Wilson, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received January 12, 2006
Revised March 1, 2006
Accepted March 2, 2006

Brief report

Induction of lethal photosensitization in biofilms using a confocal scanning laser as the excitation source

C. K. Hope 1 * and M. Wilson 2

1 School of Dental Sciences, The University of Liverpool, Liverpool, UK; Division of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, UK
2 Division of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, UK

* To whom correspondence should be addressed.
C. K. Hope, E-mail: c.hope{at}liv.ac.uk


  Abstract

Objectives: To induce lethal photosensitization in biofilms of Streptococcus pyogenes using the scanning laser in a confocal microscope to photoactivate Sn (IV) chlorin e6 (SnCe6) while simultaneously measuring changes in cell vitality using fluorescent indicators of membrane integrity.

Methods: Biofilms of S. pyogenes were immersed in a solution of 50 mg/L (70.28 µM) SnCe6 and scanned with the 488 nm argon and 543 nm HeNe lasers in a confocal microscope. Changes in membrane permeability were quantified using image analysis tools.

Results: Cell permeability increased in biofilms of S. pyogenes after successive scanning/exposure cycles in the presence of SnCe6.

Conclusions: Cell death was induced in biofilms of S. pyogenes by the photosensitizer SnCe6 on exposure to the scanning laser emissions of a confocal microscope. The simultaneous recording of cell death demonstrates the real-time evaluation of a light-activated antimicrobial compound against a biofilm.

Keywords: photodynamic therapy; Streptococcus pyogenes; Sn (IV) chlorin e6.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 JDRHome page
K. Konopka and T. Goslinski
Photodynamic Therapy in Dentistry
Journal of Dental Research, August 1, 2007; 86(8): 694 - 707.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.