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JAC Advance Access published online on March 13, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl083
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received December 29, 2005
Revised February 10, 2006
Accepted February 22, 2006

Brief report

Combinations of PBPs and MurM protein variants in early and contemporary high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain

Rosa del Campo 1 *, Fabio Cafini 2, María Isabel Morosini 1, Asunción Fenoll 3, Josefina Liñares 4, Luis Alou 2, David Sevillano 2, Rafael Cantón 1, José Prieto 2, Fernando Baquero 1, and on behalf of the Spanish Pneumococcal Network (G3/103)

1 Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Ctra. Colmenar Km 9.1, Madrid 28034, Spain
2 Departamento de Microbiología, Facultad de Medicina, Universidad Complutense, Avda Complutense s/n, Madrid 28040, Spain
3 Departamento de Microbiología, Instituto Nacional de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, Majadahonda 28220, Spain
4 Servicio de Microbiología, Hospital Universitario de Bellvitge, Feixa Llarga s/n L'Hospitalet de Llobregat, Barcelona 08097, Spain

* To whom correspondence should be addressed.
Rosa del Campo, E-mail: rosacampo{at}yahoo.com


  Abstract

Objectives: High-level penicillin resistance in Streptococcus pneumoniae requires extensive re-modulation of the penicillin-binding proteins (PBPs), and murM gene function is also required for the expression of resistance. In this work, we determined whether specific changes in PBPs were associated with specific MurM variants.

Methods: Two collections of highly penicillin-resistant (MIC 2-8 mg/L) isolates, including 10 early (1997-1998) and 23 contemporary (2002-2004) isolates, were studied.

Results: Most of the isolates belonged to clones Spain6B-2 (13 strains), Spain23F-1 (10 isolates) and Spain14-5 (20 isolates). Different protein variants of MurM (MA, MB5, MB6, MB9 and MB10), PBP1A (A-C), PBP2B (A-D) and PBP2X (A-C) were recognized, including two murM alleles not previously described. Particular [MurM-PBP1A-2B-2X] allelic combinations were predominant among the different clones, including [MA-B-B-B] for old (MIC 2 mg/L) and [MB10-C-A-B] for recent (MIC 4-8 mg/L) Spain6B-2 isolates, [MA-A-C-A] for Spain23F-1 and [MB5-A-A-A] in Spain14-5 isolates.

Conclusions: Although S. pneumoniae has a basic recombinational population structure, our results indicate remarkable conservation of PBPs and MurM protein types within each clone. This suggests that particular PBPs-MurM combinations tend to be preserved and may have an independent evolutionary history in particular clones.

Keywords: resistance; clones; mutations; penicillin-binding proteins.
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