Skip Navigation



JAC Advance Access published online on March 10, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl077
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/5/979    most recent
dkl077v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Bagattini, M.
Right arrow Articles by Zarrilli, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bagattini, M.
Right arrow Articles by Zarrilli, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received November 9, 2005
Revised February 15, 2006
Accepted February 16, 2006

Brief report

Molecular epidemiology of extended-spectrum {beta}-lactamase-producing Klebsiella pneumoniae in a neonatal intensive care unit

Maria Bagattini 1 {dagger}, Valeria Crivaro 1 {dagger}, Anna Di Popolo 1 {dagger}, Fabrizio Gentile 2, Alda Scarcella 3, Maria Triassi 1, Paolo Villari 4, and Raffaele Zarrilli 5 *

1 Dipartimento di Scienze Mediche Preventive, Sezione di Igiene, Università di Napoli ‘Federico II’, Napoli, Italy
2 Dipartimento di Scienze per la Salute, Università del Molise, Campobasso, Italy
3 Dipartimento di Pediatria, Università di Napoli ‘Federico II’, Napoli, Italy
4 Dipartimento di Medicina Sperimentale e Patologia, Università di Roma ‘La Sapienza’, Roma, Italy
5 Dipartimento di Scienze Mediche Preventive, Sezione di Igiene, Università di Napoli ‘Federico II’, Napoli, Italy; CEINGE Biotecnologie Avanzate, Napoli, Italy

* To whom correspondence should be addressed.
Raffaele Zarrilli, E-mail: rafzarri{at}unina.it


  Abstract

Objectives: To investigate the molecular epidemiology of extended-spectrum {beta}-lactamase (ESBL)-producing Klebsiella pneumoniae in the neonatal intensive care unit of a university hospital in Italy.

Methods: Antibiotic susceptibility was evaluated by disc diffusion and Etest. ESBLs were identified by isoelectric focusing, PCR and DNA sequencing analysis. Genotyping was performed by PFGE analysis. Conjugation was performed by broth mating.

Results: Molecular typing of K. pneumoniae isolates identified three distinct PFGE patterns. Isolates of PFGE profile A were isolated during an epidemic in 1996, while isolates of PFGE profiles B and C were sequentially isolated from September 2002 to December 2004, when 233 colonizations and 19 infections by K. pneumoniae occurred. All K. pneumoniae strains of different PFGE types were identified as ESBL producers. DNA sequencing of amplified {beta}-lactamase genes identified a novel blaTEM ESBL (blaTEM-136) along with blaSHV-1 in chromosomal and plasmid DNA from K. pneumoniae of PFGE type A, respectively, and blaTEM-1 and blaSHV-12 in plasmid DNA from K. pneumoniae of PFGE types B and C. Conjugation experiments demonstrated that resistance to third-generation cephalosporins, along with an ~80 kb plasmid containing blaSHV-12 and blaTEM-1, was transferred from K. pneumoniae epidemic strains of PFGE types B and C to a susceptible Escherichia coli host at a frequency of 4 x 10-6 and 1 x 10-6 cfu/recipient cell, respectively.

Conclusions: The selection of ESBL-producing clones and the transfer of the blaSHV-12 ESBL gene between different clones were responsible for the spread of K. pneumoniae in the neonatal intensive care unit.

Keywords: nosocomial infections; antimicrobial resistance; genotyping; horizontal gene transfer.
{dagger}These authors contributed equally to this work.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Trop PediatrHome page
S. Shakil, S. Z. Ali, M. Akram, S. M. Ali, and A. U. Khan
Risk Factors for Extended-Spectrum {beta}-Lactamase Producing Escherichia Coli and Klebsiella Pneumoniae Acquisition in a Neonatal Intensive Care Unit
J Trop Pediatr, July 16, 2009; (2009) fmp060v1.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
R. Zarrilli, D. Vitale, A. Di Popolo, M. Bagattini, Z. Daoud, A. U. Khan, C. Afif, and M. Triassi
A Plasmid-Borne blaOXA-58 Gene Confers Imipenem Resistance to Acinetobacter baumannii Isolates from a Lebanese Hospital
Antimicrob. Agents Chemother., November 1, 2008; 52(11): 4115 - 4120.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
A. Valverde, T. M. Coque, L. Garcia-San Miguel, F. Baquero, and R. Canton
Complex molecular epidemiology of extended-spectrum -lactamases in Klebsiella pneumoniae: a long-term perspective from a single institution in Madrid
J. Antimicrob. Chemother., January 1, 2008; 61(1): 64 - 72.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
J. Raymond, P. Nordmann, C. Doit, H. Vu Thien, M. Guibert, A. Ferroni, and Y. Aujard
Multidrug-Resistant Bacteria in Hospitalized Children: A 5-Year Multicenter Study
Pediatrics, April 1, 2007; 119(4): e798 - e803.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.