JAC Advance Access published online on February 7, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl021
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1 Division of Oral Biology, Leeds Dental Institute, University of Leeds, Clarendon Way, Leeds LS2 9LU, UK
* To whom correspondence should be addressed. Objectives: The purpose of this study was to evaluate the clinical plaque disclosing agent erythrosine as a photosensitizer in the photodynamic killing of the oral bacterium Streptococcus mutans grown as a biofilm. Methods: S. mutans biofilms of 200 µm thickness were grown in a constant-depth film fermenter. In addition to determining localization of the photosensitizer within biofilms using confocal laser scanning microscopy (CLSM), we compared the bacterial killing efficacy of erythrosine with that of two well-characterized photosensitizers, methylene blue (MB) and photofrin. Incubations were carried out with each photosensitizer (22 µM), and irradiation was for 15 min using a 400 W white light source. Results: The CLSM results showed that erythrosine is taken up into S. mutans biofilms, where it is associated with the biomass of the biofilm rather than the fluid-filled channels and voids. Comparison of the cell killing efficacy of erythrosine in S. mutans biofilms of different ages showed that erythrosine was 1-2 log10 more effective at killing biofilm bacteria than photofrin and 0.5-1 log10 more effective than MB. The results were statistically significant (P < 0.01). Photodynamic therapy (PDT) with all three photosensitizers was increasingly effective as biofilm age increased, suggesting that temporal changes in biofilm architecture and composition affect susceptibility to PDT. Conclusions: PDT using erythrosine as photosensitizer shows excellent potential as a treatment for oral plaque biofilms.
Received October 25, 2005
Revised January 13, 2006
Accepted January 16, 2006
Original article
Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms
Simon Wood 1 *,
Daniel Metcalf 2,
Deirdre Devine 1,
and
Colin Robinson 1
2 Division of Oral Biology, Leeds Dental Institute, University of Leeds, Clarendon Way, Leeds LS2 9LU, UK; Present address. Continuous Improvement, Johnson & Johnson Wound Management, Airebank Mill, Skipton, North Yorkshire BD23 3RX, UK
Simon Wood, E-mail: s.r.wood{at}leeds.ac.uk
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