JAC Advance Access published online on February 14, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl019
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, 88 JieFang Rd, Hangzhou 310009, China
* To whom correspondence should be addressed. Objectives: To determine the distribution and heterogeneity of metallo- Methods: Clinical C. meningosepticum isolates (n = 170) were collected from hospitals in Hangzhou, China. Production of MBLs was investigated by determination of imipenem MICs, and by using both a three-dimensional test and a 2-mercaptopropionic acid inhibitory test. Genes encoding BlaB and GOB MBLs were amplified by PCR, sequenced and compared with genes in GenBank. Results: More than 95% of the 170 isolates showed high (MIC > 16 mg/L) or intermediate resistance to imipenem, but only 94 isolates (55%) were shown phenotypically to produce MBLs (imipenem MIC range, 8-256 mg/L), with MBL genes detected in 93 of these. Among them, 83 isolates had blaB alleles and 65 isolates had blaGOB alleles; 38 isolates possessed one MBL gene and 55 isolates contained two genes. The major blaB alleles encoded BlaB-2, -3 and -11, while the major blaGOB alleles encoded GOB-2, -4, -8 and -10. MBLs or their genes were not detected in 76 (45%) isolates, including many that were highly resistant to imipenem. Conclusions: High levels and rates of imipenem resistance in C. meningosepticum from Hangzhou often result from the presence of heterogeneous BlaB and/or GOB MBLs, although undefined carbapenem resistance mechanisms also exist. Susceptibility testing and screening for MBLs should be conducted in order to inform effective treatment for C. meningosepticum infections.
Received November 15, 2005
Revised January 11, 2006
Accepted January 13, 2006
Brief report
Heterogeneity of metallo-
Gong-Xiang Chen 1 *,
Rong Zhang 1,
and
Hong Wei Zhou 1
-lactamases in clinical isolates of Chryseobacterium meningosepticum from Hangzhou, China
Gong-Xiang Chen, E-mail: chengong218{at}163.com
Abstract 
-lactamases (MBLs) responsible for imipenem resistance in Chryseobacterium meningosepticum.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. C. Cai, H. W. Zhou, R. Zhang, and G.-X. Chen Emergence of Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli Isolates Possessing the Plasmid-Mediated Carbapenem-Hydrolyzing {beta}-Lactamase KPC-2 in Intensive Care Units of a Chinese Hospital Antimicrob. Agents Chemother., June 1, 2008; 52(6): 2014 - 2018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Zhang, L. Yang, J. C. Cai, H. W. Zhou, and G.-X. Chen High-level carbapenem resistance in a Citrobacter freundii clinical isolate is due to a combination of KPC-2 production and decreased porin expression J. Med. Microbiol., March 1, 2008; 57(3): 332 - 337. [Abstract] [Full Text] [PDF]
|
|