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JAC Advance Access published online on January 30, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl011
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received October 8, 2005
Revised January 5, 2006
Accepted January 5, 2006

Brief report

Resistance to a polyquaternium-1 lens care solution and isoelectric points of Pseudomonas aeruginosa strains

Gerda M. Bruinsma 1, Minie Rustema-Abbing 1, Henny C. van der Mei 1 *, Carol Lakkis 2, and Henk J. Busscher 1

1 Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands
2 Clinical Vision Research Australia, The University of Melbourne, Cnr Keppel and Cardigan Streets, Carlton VIC 3053 Australia; Department of Optometry and Vision Sciences, The University of Melbourne, Australia

* To whom correspondence should be addressed.
Henny C. van der Mei, E-mail: h.c.van.der.mei{at}med.umcg.nl


  Abstract

Objectives: The aim of this study was to correlate the cell surface hydrophobicity and charge of various strains of Pseudomonas aeruginosa with their resistance to a polyquaternium-1 lens care solution.

Methods: The 11 P. aeruginosa strains included were isolated from eyes, contact lenses, lens cases and lens care solutions. Cell surface hydrophobicities were determined from water contact angle measurements and surface charges were measured as a function of pH using particulate micro-electrophoresis.

Results: Strains resistant to polyquaternium-1 had an isoelectric point (IEP; pH where the bacterial zeta potential is zero) ranging from 4.0 to 5.5, whereas susceptible strains were more negatively charged than resistant strains and had an IEP between 1.3 and 1.9. Water contact angles ranged from hydrophilic (34°) to hydrophobic (124°), without showing a relation with antimicrobial resistance.

Conclusions: Results suggest that electrostatic repulsion between cationic molecules on the cell surface and quaternary ammonium compounds impedes the antimicrobial entering the cell.

Keywords: cationic molecules; quaternary ammonium compounds; hydrophobicity; surface charge.
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