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JAC Advance Access published online on January 25, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkl003
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received August 4, 2005
Revised December 6, 2005
Accepted December 23, 2005

Brief report

Activity of human {beta}-defensins 2 and 3 against ESBL-producing Klebsiella strains

Hany Sahly 1 *, Sabine Schubert 1, Jürgen Harder 2, Michael Kleine 3, Dorthe Sandvang 4, Uwe Ullmann 1, Jens M. Schröder 2, and Rainer Podschun 1

1 Institute of Infection Medicine, Faculty of Medicine, University of Kiel, Brunswiker Strasse 4, 24105 Kiel, Germany
2 Clinical Research Unit, Department of Dermatology, Medical School, University of Kiel, Schittenhelmstrasse 7, 24105 Kiel, Germany
3 PLANTON GmbH, am Kiel-Kanal 44, 24106 Kiel, Germany
4 National Centre for Antimicrobials and Infection Control, Statens Serum Institut, Artillerivej 5, Copenhagen, Denmark

* To whom correspondence should be addressed.
Hany Sahly, E-mail: sahly{at}infmed.uni-kiel.de


  Abstract

Objectives: To test the bactericidal activity of human {beta}-defensins (hBDs) 2 and 3 against extended-spectrum {beta}-lactamase (ESBL)-producing Klebsiella strains.

Methods: Thirty-six Klebsiella pneumoniae and seventeen Klebsiella oxytoca ESBL-producing isolates from nosocomial infections were tested. The bactericidal activity of recombinantly synthesized hBD-2 and-3 was tested and the results were given either as lethal doses killing ≥90% of bacteria (LD90s) or as MBCs (≥99.9% killing).

Results: Except for one intermediately susceptible strain (MBC = 25 mg/L), all other ESBL-producing strains were highly susceptible to both defensins (LD90s and MBCs ≤ 12.5mg/L).

Conclusions: The results underline the high efficacy of hBD-2 and-3 against ESBL-producing Klebsiella, making both defensins attractive candidates as antimicrobial agents to combat these increasingly troublesome bacteria.

Keywords: multidrug resistance; innate immunity; Klebsiella spp..
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