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JAC Advance Access published online on January 5, 2006
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki478
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received August 29, 2005
Revised November 16, 2005
Accepted December 12, 2005

Original article

Distribution of a transposon-like element carrying blaCMY-2 among Salmonella and other Enterobacteriaceae

Lin-Hui Su 1, Hsiu-Ling Chen 2, Ju-Hsin Chia 1, Shu-Ying Liu 3, Chishih Chu 4, Tsu-Lan Wu 1, and Cheng-Hsun Chiu 5 *

1 Department of Clinical Pathology, Chang Gung Memorial Hospital, 5 Fu-Hsin Street, Kweishan, Taoyuan 333, Taiwan; Department of Medical Biotechnology and Laboratory Science, Chang Gung University, 259 Wenhua 1st Road, Kweishan, Taoyuan 333, Taiwan
2 Department of Pediatrics, Chang Gung Children's Hospital, 5 Fu-Hsin Street, Kweishan, Taoyuan 333, Taiwan
3 Department of Molecular Biotechnology, Da-Yeh University, 112 Shan-Jiau Road, Da-Tsuen, Changhua 515, Taiwan
4 Department of Applied Microbiology, National Chiayi University, 300 University Road, Chiayi 600, Taiwan
5 Department of Medicine, Chang Gung University, 259 Wenhua 1st Road, Kweishan, Taoyuan 333, Taiwan; Department of Pediatrics, Chang Gung Children's Hospital, 5 Fu-Hsin Street, Kweishan, Taoyuan 333, Taiwan

* To whom correspondence should be addressed.
Cheng-Hsun Chiu, E-mail: chchiu{at}adm.cgmh.org.tw


  Abstract

Objectives: The dissemination of cephamycin resistance in Enterobacteriaceae and its correlation with a transposon-like DNA element consisting of a specific tnpA-blaCMY-2-blc-sugE structure were investigated.

Methods: A total of 140 enterobacterial isolates belonging to 17 species (10 genera) of Enterobacteriaceae phenotypically characterized as putative AmpC-producers were evaluated. The isolates were examined by PCR analysis, DNA-DNA hybridization and nucleotide sequencing.

Results: The blaCMY-2-carrying element was detected in 34 isolates from 10 species (9 genera), including all 14 Salmonella and 4 Shigella isolates as well as 7 of the 10 Escherichia coli isolates tested. The remaining 9 isolates were from 112 isolates of the other 14 species tested. The genetic structure of the blaCMY-2-carrying element was identical in 29 isolates, while in 3 E. coli and 2 Citrobacter isolates an additional insertion sequence IS1 was found inserted at various nucleotide positions close to the 3' end, either within or downstream, of tnpA. In 12 of the 14 representative isolates examined, the blaCMY-2-carrying element was found inserted in the finQ gene of various-sized plasmids with highly conserved 8 bp direct repeats flanking the junction regions. Among the other 106 non-CMY-2-producing isolates, plasmid-mediated ampC genes were found only in one isolate of Enterobacter aerogenes which carried a blaDHA-1-like gene.

Conclusions: blaCMY-2 is the most prevalent plasmid-mediated ampC gene among Enterobacteriaceae. All the blaCMY-2 genes identified in the present study were associated with a specific transposon-like element that may be responsible for the spread of blaCMY-2 among Enterobacteriaceae.

Keywords: plasmid-mediated AmpC {beta}-lactamases; cephamycin resistance; DHA-1-like {beta}-lactamases.
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