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JAC Advance Access published online on December 13, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki453
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received June 22, 2005
Revised November 11, 2005
Accepted November 15, 2005

Original article

{beta}-Lactam resistance and {beta}-lactamase expression in clinical Stenotrophomonas maltophilia isolates having defined phylogenetic relationships

Virginia C. Gould 1, Aki Okazaki 1, and Matthew B. Avison 1 *

1 Bristol Centre for Antimicrobial Research and Evaluation, Department of Cellular and Molecular Medicine, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK

* To whom correspondence should be addressed.
Matthew B. Avison, E-mail: Matthewb.Avison{at}bris.ac.uk


  Abstract

Aims: To test the hypothesis that Stenotrophomonas maltophilia isolates from certain phylogenetic groups have predictable {beta}-lactamase expression and {beta}-lactam resistance profiles.

Methods: Isolates were grouped using sequences of the 16S rRNA gene and smeT-smeD intergenic region. {beta}-Lactamase activities in cell extracts were quantified spectrophotometrically and {beta}-lactam MICs were determined using agar dilution methodology and Etest as appropriate.

Results: A collection of 50 clinical S. maltophilia isolates from Europe and North, South and Central America were phylogenetically grouped. Group ‘A’ (22 out of 50) includes remarkably genetically homogeneous isolates; group ‘B’ (17 out of 50) includes isolates that are genetically heterogeneous and quite distinct from those of group A. Members of these two groups are, however, indistinguishable in terms of their {beta}-lactam resistance and {beta}-lactamase expression phenotypes. In contrast, isolates from group ‘C’, which are less common (8 out of 50), are considerably more susceptible to {beta}-lactams owing to reduced inducibility of {beta}-lactamase expression following {beta}-lactam challenge.

Conclusions: The majority of S. maltophilia clinical isolates behave similarly in terms of {beta}-lactamase expression and {beta}-lactam resistance properties, despite considerable phylogenetic variability.

Keywords: expression of resistance; bacterial diversity; in vitro susceptibility.
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