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JAC Advance Access published online on December 6, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki432
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received March 4, 2005
Revised June 22, 2005
Accepted November 1, 2005

Brief report

Use of a new alginate film test to study the bactericidal efficacy of the high-level disinfectant ortho-phthalaldehyde

J. C. N. Shackelford 1, G. W. Hanlon 1, and J.-Y. Maillard 2 *

1 School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton BN2 4GJ, UK
2 Welsh School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Avenue, Cardiff CF10 3XL, UK

* To whom correspondence should be addressed.
J.-Y. Maillard, E-mail: maillardj{at}cardiff.ac.uk


  Abstract

Objectives: To evaluate the merit of a new alginate efficacy film test to determine the bactericidal activity of the high-level disinfectant ortho-phthalaldehyde (OPA).

Methods: The efficacy of OPA was investigated using a new sodium alginate surface film test against Mycobacterium chelonae NCIMB 1474 and Epping, and Pseudomonas aeruginosa NCIMB 10421 under different test conditions.

Results: OPA was highly bactericidal against P. aeruginosa but its mycobactericidal efficacy was seriously reduced and produced ≥5 log reductions only at a concentration of 0.5% (w/v) within 30-60 min without organic load.

Conclusions: The sodium alginate film efficacy was reproducible between repeats. Inactivation results depended upon the concentration of OPA, contact time, the presence of an organic load and the bacterial genera.

Keywords: disinfection; time-kill assay; mycobacteria; Pseudomonas aeruginosa.
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