JAC Advance Access published online on October 21, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki395
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Servei de Microbiologia de l'Hospital de la Santa Creu i Sant Pau, Barcelona, Spain
* To whom correspondence should be addressed. Objectives: The aim of the present study was to characterize and compare the extended-spectrum Methods: A total of 5251 Enterobacteriaceae isolated from clinical samples and 1321 stool samples were evaluated for the presence of ESBLs. The stool samples were spread onto plates of MacConkey agar containing 2 mg/L cefotaxime for selection of ESBL-producing strains. These strains were defined as those showing synergism between amoxicillin/clavulanic acid and third-generation cephalosporins. The Results: The prevalence of ESBL-producing strains among clinical Enterobacteriaceae was 1.7%. Of these, 87.6% produced CTX-M, 25.8% produced SHV and 2.2% were TEM-type-producing strains. All clinical ESBL-producing strains were Escherichia coli, with the exception of four Klebsiella pneumoniae and one Citrobacter freundii. The prevalence of faecal carriage of ESBL-producing organisms was 3.3%. Of these, 75% produced CTX-M-type enzymes followed by 22.7% SHV-producing strains. All faecal ESBL-producing strains were E. coli except for one Enterobacter cloacae and one Proteus mirabilis. This latter strain produced the PER-1 enzyme reported for the first time in Spain. Conclusions: The prevalence of ESBL-producing strains in stool samples was higher than that observed in clinical samples from the same period. The different types of ESBLs found were similar in both contexts. The most prevalent ESBLs were the CTX-M-related enzymes, with nine different types, followed by SHV-12.
Received July 29, 2005
Revised September 21, 2005
Accepted October 3, 2005
Brief report
Surveillance of extended-spectrum
-lactamases from clinical samples and faecal carriers in Barcelona, Spain
2 Servei de Microbiologia de l'Hospital de la Santa Creu i Sant Pau, Barcelona, Spain; Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain
3 Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain
Ferran Navarro, E-mail: fnavarror{at}santpau.es
Abstract 
-lactamase (ESBL)-producing organisms isolated from clinical samples and faecal carriers in 2001 and 2002.-lactamases involved were characterized by isoelectric focusing, PCR assays and DNA sequencing.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Schneider, A. M. Queenan, R. Markovska, B. Markova, E. Keuleyan, and A. Bauernfeind New Variant of CTX-M-Type Extended-Spectrum {beta}-Lactamases, CTX-M-71, with a Gly238Cys Substitution in a Klebsiella pneumoniae Isolate from Bulgaria Antimicrob. Agents Chemother., October 1, 2009; 53(10): 4518 - 4521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ruppe, P.-L. Woerther, A. Diop, A.-M. Sene, A. Da Costa, G. Arlet, A. Andremont, and B. Rouveix Carriage of CTX-M-15-Producing Escherichia coli Isolates among Children Living in a Remote Village in Senegal Antimicrob. Agents Chemother., July 1, 2009; 53(7): 3135 - 3137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rodriguez-Bano, J. Alcala, J. M. Cisneros, F. Grill, A. Oliver, J. P. Horcajada, T. Tortola, B. Mirelis, G. Navarro, M. Cuenca, et al. Escherichia coli producing SHV-type extended-spectrum {beta}-lactamase is a significant cause of community-acquired infection J. Antimicrob. Chemother., April 1, 2009; 63(4): 781 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rodriguez-Bano, L. Lopez-Cerero, M. D. Navarro, P. D. de Alba, and A. Pascual Faecal carriage of extended-spectrum {beta}-lactamase-producing Escherichia coli: prevalence, risk factors and molecular epidemiology J. Antimicrob. Chemother., November 1, 2008; 62(5): 1142 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Calatayud, M. Arnan, J. Linares, M. A. Dominguez, C. Gudiol, J. Carratala, M. Batlle, J. M. Ribera, and F. Gudiol Prospective Study of Fecal Colonization by Extended-Spectrum-{beta}-Lactamase-Producing Escherichia coli in Neutropenic Patients with Cancer Antimicrob. Agents Chemother., November 1, 2008; 52(11): 4187 - 4190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lopez-Cerero, M. De Cueto, C. Saenz, D. Navarro, C. Velasco, J. Rodriguez-Bano, and A. Pascual Neonatal sepsis caused by a CTX-M-32-producing Escherichia coli isolate J. Med. Microbiol., October 1, 2008; 57(10): 1303 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lavilla, J. J. Gonzalez-Lopez, E. Miro, A. Dominguez, M. Llagostera, R. M. Bartolome, B. Mirelis, F. Navarro, and G. Prats Dissemination of extended-spectrum {beta}-lactamase-producing bacteria: the food-borne outbreak lesson J. Antimicrob. Chemother., June 1, 2008; 61(6): 1244 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Aragon, B. Mirelis, E. Miro, C. Mata, L. Gomez, A. Rivera, P. Coll, and F. Navarro Increase in {beta}-lactam-resistant Proteus mirabilis strains due to CTX-M- and CMY-type as well as new VEB- and inhibitor-resistant TEM-type {beta}-lactamases J. Antimicrob. Chemother., May 1, 2008; 61(5): 1029 - 1032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Madec, C. Lazizzera, P. Chatre, D. Meunier, S. Martin, G. Lepage, M.-F. Menard, P. Lebreton, and T. Rambaud Prevalence of Fecal Carriage of Acquired Expanded-Spectrum Cephalosporin Resistance in Enterobacteriaceae Strains from Cattle in France J. Clin. Microbiol., April 1, 2008; 46(4): 1566 - 1567. [Full Text] [PDF]
|
|
|
|
|
|
J. Lee, H. Pai, Y. K. Kim, N. H. Kim, B. W. Eun, H. J. Kang, K. H. Park, E. H. Choi, H. Y. Shin, E. C. Kim, et al. Control of extended-spectrum {beta}-lactamase-producing Escherichia coli and Klebsiella pneumoniae in a children's hospital by changing antimicrobial agent usage policy J. Antimicrob. Chemother., September 1, 2007; 60(3): 629 - 637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Empel, K. Filczak, A. Mrowka, W. Hryniewicz, D. M. Livermore, and M. Gniadkowski Outbreak of Pseudomonas aeruginosa Infections with PER-1 Extended-Spectrum {beta}-Lactamase in Warsaw, Poland: Further Evidence for an International Clonal Complex J. Clin. Microbiol., September 1, 2007; 45(9): 2829 - 2834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Girlich, L. Poirel, A. Carattoli, I. Kempf, M.-F. Lartigue, A. Bertini, and P. Nordmann Extended-Spectrum {beta}-Lactamase CTX-M-1 in Escherichia coli Isolates from Healthy Poultry in France Appl. Envir. Microbiol., July 15, 2007; 73(14): 4681 - 4685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Ensor, M. Shahid, J. T. Evans, and P. M. Hawkey Occurrence, prevalence and genetic environment of CTX-M {beta}-lactamases in Enterobacteriaceae from Indian hospitals J. Antimicrob. Chemother., December 1, 2006; 58(6): 1260 - 1263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Oteo, C. Navarro, E. Cercenado, A. Delgado-Iribarren, I. Wilhelmi, B. Orden, C. Garcia, S. Miguelanez, M. Perez-Vazquez, S. Garcia-Cobos, et al. Spread of Escherichia coli Strains with High-Level Cefotaxime and Ceftazidime Resistance between the Community, Long-Term Care Facilities, and Hospital Institutions. J. Clin. Microbiol., July 1, 2006; 44(7): 2359 - 2366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Mesa, V. Blanc, A. R. Blanch, P. Cortes, J. J. Gonzalez, S. Lavilla, E. Miro, M. Muniesa, M. Saco, M. T. Tortola, et al. Extended-spectrum {beta}-lactamase-producing Enterobacteriaceae in different environments (humans, food, animal farms and sewage) J. Antimicrob. Chemother., July 1, 2006; 58(1): 211 - 215. [Abstract] [Full Text] [PDF]
|
|