JAC Advance Access published online on October 14, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki371
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 K.-Bicêtre Cedex, France
* To whom correspondence should be addressed. Objectives: To gain insight into the functionality of qnr-like genes of several bacterial species of Vibrionaceae that may encode quinolone resistance determinants. Methods: A PCR-based strategy was used to obtain qnr-like genes of reference strains of Vibrio vulnificus CIP103196, Vibrio parahaemolyticus CIP71.2 and Photobacterium profundum CIP106289 that were sequenced, cloned and expressed in Escherichia coli. MICs of quinolones were determined for these reference strains and an E. coli reference strain harbouring recombinant plasmids. Results: The Qnr-like proteins of these Vibrionaceae bacterial species shared 40-67% amino acid identity with the plasmid-mediated quinolone resistance determinants QnrA, QnrB and QnrS with a series of highly conserved residues. Once cloned in E. coli they conferred reduced susceptibility to quinolones. Conclusions: This work provides further evidence that water-borne Vibrionaceae may constitute a reservoir for Qnr-like quinolone resistance determinants.
Received May 19, 2005
Accepted September 15, 2005
Brief report
Vibrionaceae as a possible source of Qnr-like quinolone resistance determinants
2 Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 K.-Bicêtre Cedex, France; University Hospital Virgen Macarena, University of Sevilla, Sevilla, Spain
Patrice Nordmann, E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Strahilevitz, G. A. Jacoby, D. C. Hooper, and A. Robicsek Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat Clin. Microbiol. Rev., October 1, 2009; 22(4): 664 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Rodriguez-Martinez, A. Briales, C. Velasco, M. C. Conejo, L. Martinez-Martinez, and A. Pascual Mutational analysis of quinolone resistance in the plasmid-encoded pentapeptide repeat proteins QnrA, QnrB and QnrS J. Antimicrob. Chemother., June 1, 2009; 63(6): 1128 - 1134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Cavaco, H. Hasman, S. Xia, and F. M. Aarestrup qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin Antimicrob. Agents Chemother., February 1, 2009; 53(2): 603 - 608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wang, G. A. Jacoby, D. M. Mills, and D. C. Hooper SOS Regulation of qnrB Expression Antimicrob. Agents Chemother., February 1, 2009; 53(2): 821 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Cattoir, P. Nordmann, J. Silva-Sanchez, P. Espinal, and L. Poirel ISEcp1-Mediated Transposition of qnrB-Like Gene in Escherichia coli Antimicrob. Agents Chemother., August 1, 2008; 52(8): 2929 - 2932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lascols, I. Podglajen, C. Verdet, V. Gautier, L. Gutmann, C.-J. Soussy, E. Collatz, and E. Cambau A Plasmid-Borne Shewanella algae Gene, qnrA3, and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae J. Bacteriol., August 1, 2008; 190(15): 5217 - 5223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Jacoby, V. Cattoir, D. Hooper, L. Martinez-Martinez, P. Nordmann, A. Pascual, L. Poirel, and M. Wang qnr Gene Nomenclature Antimicrob. Agents Chemother., July 1, 2008; 52(7): 2297 - 2299. [Full Text] [PDF]
|
|
|
|
|
|
J. M. Rodriguez-Martinez, C. Velasco, A. Briales, I. Garcia, M. C. Conejo, and A. Pascual Qnr-like pentapeptide repeat proteins in Gram-positive bacteria J. Antimicrob. Chemother., June 1, 2008; 61(6): 1240 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Quiroga, P. Andres, A. Petroni, A. J. C. Soler Bistue, L. Guerriero, L. J. Vargas, A. Zorreguieta, M. Tokumoto, C. Quiroga, M. E. Tolmasky, et al. Complex Class 1 Integrons with Diverse Variable Regions, Including aac(6')-Ib-cr, and a Novel Allele, qnrB10, Associated with ISCR1 in Clinical Enterobacterial Isolates from Argentina Antimicrob. Agents Chemother., December 1, 2007; 51(12): 4466 - 4470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Arsene and R. Leclercq Role of a qnr-Like Gene in the Intrinsic Resistance of Enterococcus faecalis to Fluoroquinolones Antimicrob. Agents Chemother., September 1, 2007; 51(9): 3254 - 3258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Cattoir, L. Poirel, D. Mazel, C.-J. Soussy, and P. Nordmann Vibrio splendidus as the Source of Plasmid-Mediated QnrS-Like Quinolone Resistance Determinants Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2650 - 2651. [Full Text] [PDF]
|
|
|
|
|
|
D. J. Colquhoun, L. Aarflot, and C. F. Melvold gyrA and parC Mutations and Associated Quinolone Resistance in Vibrio anguillarum Serotype O2b Strains Isolated from Farmed Atlantic Cod (Gadus morhua) in Norway Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2597 - 2599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Lavigne, H. Marchandin, J. Delmas, N. Bouziges, E. Lecaillon, L. Cavalie, H. Jean-Pierre, R. Bonnet, and A. Sotto qnrA in CTX-M-Producing Escherichia coli Isolates from France Antimicrob. Agents Chemother., December 1, 2006; 50(12): 4224 - 4228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Garnier, N. Raked, A. Gassama, F. Denis, and M.-C. Ploy Genetic Environment of Quinolone Resistance Gene qnrB2 in a Complex sul1-Type Integron in the Newly Described Salmonella enterica Serovar Keurmassar. Antimicrob. Agents Chemother., September 1, 2006; 50(9): 3200 - 3202. [Abstract] [Full Text] [PDF]
|
|