Skip Navigation



JAC Advance Access published online on October 21, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki369
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/6/1111    most recent
dki369v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Choi, S.-H.
Right arrow Articles by Kim, Y. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Choi, S.-H.
Right arrow Articles by Kim, Y. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received May 16, 2005
Revised September 14, 2005
Accepted September 15, 2005

Brief report

Increasing incidence of quinolone resistance in human non-typhoid Salmonella enterica isolates in Korea and mechanisms involved in quinolone resistance

Sang-Ho Choi 1, Jun Hee Woo 2, Jung Eun Lee 2, Su Jin Park 2, Eun Ju Choo 2, Yee Gyung Kwak 2, Mi-Na Kim 3, Myung-Sik Choi 4, Nam Yong Lee 5, Bok Kwon Lee 6, Nam Joong Kim 2, Jin-Yong Jeong 2, Jiso Ryu 2, and Yang Soo Kim 2*

1 Division of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea; Center for Antimicrobial Resistance and Microbial Genetics, University of Ulsan, Seoul, Republic of Korea; Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Republic of Korea
2 Division of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea; Center for Antimicrobial Resistance and Microbial Genetics, University of Ulsan, Seoul, Republic of Korea
3 Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea
4 Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Republic of Korea
5 Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
6 Department of Microbiology, Korea Center for Disease Control and Prevention, Seoul, Republic of Korea

* To whom correspondence should be addressed.
Yang Soo Kim, E-mail: yskim{at}amc.seoul.kr


  Abstract

Objectives: We investigated the trends of nalidixic acid resistance in human non-typhoid Salmonella enterica in a Korean population, and examined some possible mechanisms involved in this resistance.

Methods: A total of 261 clinical strains were tested. For all strains, the MICs of nalidixic acid were determined. Nalidixic acid-resistant strains underwent further analysis, including determination of MICs of other antibiotics, mutation analysis within the topoisomerase genes, organic solvent tolerance test, western blotting for AcrA, marOR mutation analysis, ciprofloxacin accumulation test, and PCR for the qnr gene. The clonal relationships of Salmonella strains were examined by random amplified polymorphic DNA analysis.

Results: The incidence of nalidixic acid resistance increased from 1.8% in 1995-96 to 21.8% in 2000-02. The resistance rate was higher in S. enterica serotype Enteritidis (21.6%) than in serotype Typhimurium (12.1%). The nalidixic acid resistance rates in Salmonella Enteritidis varied according to the phage type (PT) and Salmonella Enteritidis PT 1 was most commonly associated with resistance to nalidixic acid. Several cases of clonal spread, especially by Salmonella Enteritidis PT 1, were identified. Of the 46 nalidixic acid-resistant strains, 43 had single mutations in the gyrA gene. Four strains were organic solvent-tolerant and were associated with decreased ciprofloxacin accumulation; three of these showed increased expression of AcrA and had novel mutations in marOR (I84L). The qnr gene was not identified.

Conclusions: Recently, the rate of nalidixic acid resistance in Korean clinical Salmonella strains markedly increased and it was partly due to the clonal spread of Salmonella Enteritidis, especially PT 1. The main mechanism of nalidixic acid resistance was a mutation in the gyrA region.

Keywords: S. enterica; nalidixic acid; drug resistance.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Evid Based Complement Alternat MedHome page
J.-G. Choi, O.-H. Kang, Y.-S. Lee, H.-S. Chae, Y.-C. Oh, O.-O. Brice, M.-S. Kim, D.-H. Sohn, H.-S. Kim, H. Park, et al.
In vitro and In vivo Antibacterial Activity of Punica granatum Peel Ethanol Extract Against Salmonella
Evid. Based Complement. Altern. Med., August 17, 2009; (2009) nep105v1.
[Abstract] [Full Text] [PDF]

Home page
 Vet Rec.Home page
J. J. Carrique-Mas, C. Papadopoulou, S. J. Evans, A. Wales, C. J. Teale, and R. H. Davies
Trends in phage types and antimicrobial resistance of Salmonella enterica serovar Enteritidis isolated from animals in Great Britain from 1990 to 2005
Vet Rec., April 26, 2008; 162(17): 541 - 546.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
J. Guard-Bouldin, C. A. Morales, J. G. Frye, R. K. Gast, and M. Musgrove
Detection of Salmonella enterica Subpopulations by Phenotype Microarray Antibiotic Resistance Patterns
Appl. Envir. Microbiol., December 1, 2007; 73(23): 7753 - 7756.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
H. Solnik-Isaac, M. Weinberger, M. Tabak, A. Ben-David, D. Shachar, and S. Yaron
Quinolone Resistance of Salmonella enterica Serovar Virchow Isolates from Humans and Poultry in Israel: Evidence for Clonal Expansion
J. Clin. Microbiol., August 1, 2007; 45(8): 2575 - 2579.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
V. Cattoir, F.-X. Weill, L. Poirel, L. Fabre, C.-J. Soussy, and P. Nordmann
Prevalence of qnr genes in Salmonella in France
J. Antimicrob. Chemother., April 1, 2007; 59(4): 751 - 754.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.