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JAC Advance Access published online on September 29, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki350
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received March 29, 2005
Revised August 16, 2005
Accepted September 8, 2005

Brief report

Cationic peptide of the male reproductive tract, HE2{alpha}, displays antimicrobial activity against Neisseria gonorrhoeae, Staphylococcus aureus and Enterococcus faecalis

M. Liao 1, P. S. Ruddock 2, A. S. Rizvi 3, S. H. Hall 4, F. S. French 4, and J. R. Dillon 5*

1 Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON, Canada, K1H 8M5; Centre for Research in Biopharmaceuticals and Biotechnology, University of Ottawa, ON, Canada, K1H 8M5; Present address. Room A233, 120 Veterinary Road, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3
2 Department of Biology, University of Ottawa, ON, Canada, K1H 8M5
3 Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON, Canada, K1H 8M5
4 Laboratories for Reproductive Biology, University of North Carolina at Chapel Hill, Campus Box 7500, Chapel Hill, NC 27599, USA
5 Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ON, Canada, K1H 8M5; Centre for Research in Biopharmaceuticals and Biotechnology, University of Ottawa, ON, Canada, K1H 8M5; Department of Biology, University of Ottawa, ON, Canada, K1H 8M5

* To whom correspondence should be addressed.
J. R. Dillon, E-mail: j.dillon{at}usask.ca


  Abstract

Objectives: To analyse the in vitro antimicrobial effects of synthetic HE2{alpha} peptide against Neisseria gonorrhoeae, Staphylococcus aureus and Enterococcus faecalis.

Methods: The HE2{alpha} peptide was synthesized based on the C-terminal sequence of the HE2{alpha} protein. The bacterial strains tested included two antibiotic-susceptible strains of N. gonorrhoeae and four antibiotic-resistant clinical isolates, as well as S. aureus ATCC 29213 and E. faecalis ATCC 29212. Susceptibility determinations were carried out either in 0.7% casamino acids for N. gonorrhoeae isolates or in 10 mM phosphate buffer for S. aureus and E. faecalis strains. Antibacterial effects were measured in a dose- and time-dependent manner. After exposure to the peptide in solution, the number of viable cells was determined by counting colony forming units (cfu).

Results: The HE2{alpha} peptide exhibited time- and dose-dependent antibacterial effects on all N. gonorrhoeae isolates tested. S. aureus and E. faecalis strains were also susceptible to the peptide. All strains tested were susceptible to the peptide at high concentrations (50 or 100 mg/L) and some strains were susceptible to a peptide concentration of 25 mg/L.

Conclusions: The peptide HE2{alpha}, which is derived from the male urogenital tract, exhibits antibacterial activity against both Gram-positive and Gram-negative pathogens in vitro. The peptide is active against both antibiotic-susceptible and -resistant N. gonorrhoeae isolates. Further investigation of the antimicrobial properties of the peptide is warranted.

Keywords: susceptibility tests; antimicrobial peptides; sexually transmitted diseases; pathogenic bacteria.
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