JAC Advance Access published online on September 27, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki345
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1 Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain; Unitat de Microbiologia, Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
* To whom correspondence should be addressed. Objectives: The dispersion of a clone, a plasmid or a mobile element carrying the blaCTX-M-9 gene was evaluated in 30 Escherichia coli strains isolated in Barcelona between 1996 and 1999. The presence of the previously described orf513-bearing class 1 integron, In60, carrying the blaCTX-M-9 gene, was also studied. Methods: The clonality was analysed by pulsed-field gel electrophoresis. Plasmid analysis was performed by S1 digestion and hybridization with the CTX-M-9 probe. PCR mapping using specific designed primers was used to study the presence of In60 and In60-like structures. Results: The clonality between the 30 strains was minor. The size of blaCTX-M-9 carrying plasmids ranged between Conclusions: These findings suggest that the bla CTX-M-9 gene may be carried by a mobile element that disperses it between plasmids. The fast dispersion of the CTX-M-9 enzyme could therefore be due to both diffusion of plasmids and mobile elements.
Received March 24, 2005
Revised July 18, 2005
Accepted August 30, 2005
Original article
Characterization of the highly variable region surrounding the blaCTX-M-9 gene in non-related Escherichia coli from Barcelona
2 Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain
3 Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona-Institut de Recerca i Tecnologia Agroalimentària, 08193 Bellaterra, Spain
Ferran Navarro, E-mail: fnavarror{at}santpau.es
Abstract 
80 and 430 kb. One strain produced only a chromosome-encoded CTX-M-9 
-lactamase. Thirty-six per cent of the strains showed differences with respect to the In60 structure due to an insertion or deletion events.-lactamases; plasmids; integrons.
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