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JAC Advance Access published online on September 26, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki337
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received May 16, 2005
Revised August 8, 2005
Accepted August 24, 2005

Original article

Differences in the DNA sequence of the translational attenuator of several constitutively expressed erm(A) genes from clinical isolates of Streptococcus agalactiae

Esther Culebras 1*, Iciar Rodríguez-Avial 1, Carmen Betriu 1, and Juan J. Picazo 1

1 Servicio de Microbiología, Hospital Clínico San Carlos, Plaza Cristo Rey s/n, Madrid, 28040, Spain

* To whom correspondence should be addressed.
Esther Culebras, E-mail: eculebras.hcsc{at}salud.madrid.org


  Abstract

Objectives: To study the regulatory region of a constitutively expressed erm(A) gene in Streptococcus agalactiae clinical isolates.

Methods: Thirty clinical isolates of S. agalactiae which were cross-resistant to erythromycin and clindamycin and with a clindamycin MIC higher than that of erythromycin were studied by PCR, sequencing and molecular typing.

Results: PCR analysis revealed that all the strains harboured the erm(A) gene, either alone (26 isolates) or in combination with erm(B) (four isolates). Sequencing of the region upstream of erm(A) showed that all isolates possessed two types of genetic alteration. Most of the strains showed point mutations in the second leader peptide (mainly A137C) and, in four isolates (two clones), an insertion fragment with high homology to IS1381 and transposase genes was detected. Epidemiological analysis of strains indicated several clonal origins of isolates.

Conclusions: The mutations described here are thought to result in increased or constitutive expression of the erm(A) gene.

Keywords: clindamycin resistance; Group B streptococci; gene regulation.
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