Skip Navigation



JAC Advance Access published online on September 2, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki324
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/4/698    most recent
dki324v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ryoo, N. H.
Right arrow Articles by Jeong, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ryoo, N. H.
Right arrow Articles by Jeong, S. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received April 26, 2005
Revised August 15, 2005
Accepted August 15, 2005

Original article

Dissemination of SHV-12 and CTX-M-type extended-spectrum {beta}-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea

Nam Hee Ryoo 1, Eui-Chong Kim 2, Seong Geun Hong 3, Yeon Joon Park 4, Kyungwon Lee 5, Il Kwon Bae 6, Eun Hyang Song 6, and Seok Hoon Jeong 7*

1 Department of Laboratory Medicine, Keimyung University College of Medicine, Daegu, Korea
2 Seoul National University College of Medicine, Seoul, Korea
3 Pochon CHA University College of Medicine, Sungnam, Korea
4 The Catholic University of Korea College of Medicine, Seoul, Korea
5 Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea; Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea
6 Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Korea
7 Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea; Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Korea

* To whom correspondence should be addressed.
Seok Hoon Jeong, E-mail: kscpjsh{at}ns.kosinmed.or.kr


  Abstract

Objectives: To assess the prevalence and genotypes of Ambler class A extended-spectrum {beta}-lactamases (ESBLs) in Korea.

Methods: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February-July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A {beta}-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing.

Results: The double-disc synergy test showed positive results in 9.3% (23/246) of E. coli and 23.0% (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n = 4) and CTX-M-3 (n = 3), and those in K. pneumoniae isolates were SHV-12 (n = 30) and CTX-M-3 (n = 13). Two isolates produced both SHV-12 and GES-3, simultaneously.

Conclusions: CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.

Keywords: ESBLs; resistance; prevalence.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
L. Yuan, J.-H. Liu, G.-Z. Hu, Y.-S. Pan, Z.-M. Liu, J. Mo, and Y.-J. Wei
Molecular characterization of extended-spectrum {beta}-lactamase-producing Escherichia coli isolates from chickens in Henan Province, China
J. Med. Microbiol., November 1, 2009; 58(11): 1449 - 1453.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
W. Liu, L. Chen, H. Li, H. Duan, Y. Zhang, X. Liang, X. Li, M. Zou, L. Xu, and P. M. Hawkey
Novel CTX-M {beta}-lactamase genotype distribution and spread into multiple species of Enterobacteriaceae in Changsha, Southern China
J. Antimicrob. Chemother., May 1, 2009; 63(5): 895 - 900.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
W. Song, H. Lee, K. Lee, S. H. Jeong, I. K. Bae, J.-S. Kim, and H.-S. Kwak
CTX-M-14 and CTX-M-15 enzymes are the dominant type of extended-spectrum {beta}-lactamase in clinical isolates of Escherichia coli from Korea
J. Med. Microbiol., February 1, 2009; 58(2): 261 - 266.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
J. Walther-Rasmussen and N. Hoiby
Class A carbapenemases
J. Antimicrob. Chemother., September 1, 2007; 60(3): 470 - 482.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
I. K. Bae, Y.-N. Lee, W. G. Lee, S. H. Lee, and S. H. Jeong
Novel Complex Class 1 Integron Bearing an ISCR1 Element in an Escherichia coli Isolate Carrying the blaCTX-M-14 Gene
Antimicrob. Agents Chemother., August 1, 2007; 51(8): 3017 - 3019.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
A. M. Queenan and K. Bush
Carbapenemases: the Versatile {beta}-Lactamases
Clin. Microbiol. Rev., July 1, 2007; 20(3): 440 - 458.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
W. Song, I. K. Bae, Y.-N. Lee, C.-H. Lee, S. H. Lee, and S. H. Jeong
Detection of Extended-Spectrum {beta}-Lactamases by Using Boronic Acid as an AmpC {beta}-Lactamase Inhibitor in Clinical Isolates of Klebsiella spp. and Escherichia coli
J. Clin. Microbiol., April 1, 2007; 45(4): 1180 - 1184.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
I. K. Bae, Y.-N. Lee, H. Y. Hwang, S. H. Jeong, S. J. Lee, H.-S. Kwak, W. Song, H. J. Kim, and H. Youn
Emergence of CTX-M-12 extended-spectrum {beta}-lactamase-producing Escherichia coli in Korea
J. Antimicrob. Chemother., December 1, 2006; 58(6): 1257 - 1259.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
L. Poirel, T. Naas, and P. Nordmann
Pyrosequencing as a Rapid Tool for Identification of GES-Type Extended-Spectrum {beta}-Lactamases.
J. Clin. Microbiol., August 1, 2006; 44(8): 3008 - 3011.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
I. K. Bae, B. H. Lee, H. Y. Hwang, S. H. Jeong, S. G. Hong, C. L. Chang, H.-S. Kwak, H. J. Kim, and H. Youn
A novel ceftazidime-hydrolysing extended-spectrum {beta}-lactamase, CTX-M-54, with a single amino acid substitution at position 167 in the omega loop
J. Antimicrob. Chemother., August 1, 2006; 58(2): 315 - 319.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
Y. H. Lee, B. Cho, I. K. Bae, C. L. Chang, and S. H. Jeong
Klebsiella pneumoniae strains carrying the chromosomal SHV-11 {beta}-lactamase gene produce the plasmid-mediated SHV-12 extended-spectrum {beta}-lactamase more frequently than those carrying the chromosomal SHV-1 {beta}-lactamase gene
J. Antimicrob. Chemother., June 1, 2006; 57(6): 1259 - 1261.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.