JAC Advance Access first published online on July 26, 2005
This version published online on September 9, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki254
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1 Department of Laboratory Medicine, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, Japan; Group of Infection Control Research, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, Japan
* To whom correspondence should be addressed. Objectives: Pseudomonas putida is an uncommon opportunistic pathogen, usually susceptible to antimicrobial agents. Data concerning resistance to antimicrobial agents in clinical P. putida isolates are limited. Patients and methods: Susceptibilities to fluoroquinolones, carbapenems and other antibiotics were characterized in five clinical isolates of P. putida recovered from different patients with urinary tract infections as causative pathogens. Fluoroquinolone and carbapenem resistance were characterized genetically by the methods of PCR and DNA sequencing. Outer membrane protein (OMP) profiles were characterized by SDS-PAGE. Results: Four of five isolates were resistant or intermediate to both fluoroquinolones and carbapenems. Nucleotide sequences in the quinolone resistance-determining regions suggested that amino acid mutations such as Thr-83 Conclusions: This study identified mechanisms of resistance to fluoroquinolones and carbapenems in clinical P. putida isolates.
Received March 27, 2005
Revised May 30, 2005
Accepted June 23, 2005
Original article
Mechanisms of resistance to fluoroquinolones and carbapenems in Pseudomonas putida
2 Group of Infection Control Research, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, Japan; Division of Pharmacy, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, Japan
3 Department of Clinical Laboratory Medicine, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan
Toshinobu Horii, E-mail: horiihm{at}hama-med.ac.jp
Abstract 
Ile in GyrA and Glu-469Asp in GyrB may contribute to high resistance to fluoroquinolones. Four metallo-
-lactamase-producing isolates that showed resistance to carbapenems carried the IMP-type metallo--lactamase genes. A combined effect of reduced production of 46 kDa OMP and metallo--lactamase production was shown by a P. putida isolate exhibiting the highest MICs of carbapenems.-lactamases.
The orginally published version of this article was incorrect. A citation to reference 9a was omitted and reference 19 was included in error. The author apologizes for these errors.
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