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JAC Advance Access published online on July 20, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki250
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received April 22, 2005
Revised June 19, 2005
Accepted June 20, 2005

Brief report

Plasmid-mediated resistance to ciprofloxacin and cefotaxime in clinical isolates of Salmonella enterica serotype Enteritidis in Hong Kong

Terence Kin Man Cheung 1, Yiu Wai Chu 1*, Man Yu Chu 1, Choi Ha Ma 1, Raymond Wai Hung Yung 2, and Kai Man Kam 1

1 Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong SAR, China
2 Infection Control Branch, Centre for Health Protection, Department of Health, Hong Kong SAR, China

* To whom correspondence should be addressed.
Yiu Wai Chu, E-mail: alf{at}chp.gov.hk


  Abstract

Objectives: To characterize the genetic determinants involved in the reduced susceptibility to ciprofloxacin and cefotaxime in Salmonella enterica serotype Enteritidis clinical isolates obtained from four patients in summer 2003 in Hong Kong.

Methods: Three Salmonella Enteritidis isolates from blood culture and one from stool were collected due to their increased resistance to ciprofloxacin and cefotaxime. PFGE analysis was used to investigate their genetic relatedness. Conjugation experiments were employed to show if the genetic determinants involved were plasmid-mediated. MICs of various antimicrobials were determined by VITEK 2 and Etest. Based on the susceptibility and conjugation experiment results, previously described PCR methods were employed to detect sequences homologous to qnr and blaCTX-M suspected to be involved in the reduced susceptibility to ciprofloxacin and cefotaxime, respectively.

Results: PFGE analysis showed that the four Salmonella isolates were clonally unrelated. The presence of a qnr-like gene and the CTX-M allele blaCTX-M-14 on four different transferable plasmids harboured by the four isolates was confirmed.

Conclusions: This is the first report of transferable fluoroquinolone resistance due to a new qnr allele, which appeared to be linked to blaCTX-M-14, in isolates of Salmonella Enteritidis in Hong Kong.

Keywords: Qnr; CTX-M-14; extended-spectrum {beta}-lactamases; ESBLs.
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