Skip Navigation



JAC Advance Access published online on July 18, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki246
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/3/583    most recent
dki246v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by González-Zorn, B.
Right arrow Articles by Moreno, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by González-Zorn, B.
Right arrow Articles by Moreno, M. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received May 11, 2005
Revised May 26, 2005
Accepted June 15, 2005

Brief report

Genetic basis for dissemination of armA

Bruno González-Zorn 1*, Ana Catalan 1 {dagger}, Jose A. Escudero 1 {dagger}, Lucas Domínguez 1, Tirushet Teshager 1, Concepción Porrero 1, and Miguel Angel Moreno 1

1 Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain

* To whom correspondence should be addressed.
Bruno González-Zorn, E-mail: bgzorn{at}vet.ucm.es


  Abstract

Objectives and methods: armA is a novel plasmid-borne 16S rRNA methyltransferase that confers high-level resistance to 4,6-disubstituted deoxystreptamines. Recently, we have isolated from a high-level broad-spectrum aminoglycoside-resistant Escherichia coli animal isolate a plasmid, pMUR050, that bore the armA gene. In order to elucidate the genetic basis for the spread of armA, we have determined the complete nucleotide sequence of pMUR050.

Results: armA was borne by a complex transposon composite flanked by two direct repeats of IS26. The transposon composite included a class one integron with sul1 for resistance to sulphonamides and ant3''9 conferring resistance to spectinomycin-streptomycin, and a macrolide efflux pump and mefE/mel conferring high-level resistance to erythromycin. We identified in GenBank that another plasmid, pCTX-M3, from a Polish Citrobacter freundii human isolate, bore the same genetic structure, including armA.

Conclusions: armA is present in human and animal isolates within a novel transposon composite. Further spread of armA between bacteria of diverse origin is to be expected.

Keywords: Escherichia coli; animal isolates; 16S rRNA methylase; aminoglycoside resistance; IncN; transposon composite.
{dagger}These authors contributed equally to this work.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
X.-D. Du, C.-M. Wu, H.-B. Liu, X.-S. Li, R. C. Beier, F. Xiao, S.-S. Qin, S.-Y. Huang, and J.-Z. Shen
Plasmid-mediated ArmA and RmtB 16S rRNA methylases in Escherichia coli isolated from chickens
J. Antimicrob. Chemother., December 1, 2009; 64(6): 1328 - 1330.
[Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
P. D. Lister, D. J. Wolter, and N. D. Hanson
Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms
Clin. Microbiol. Rev., October 1, 2009; 22(4): 582 - 610.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J. P. Folster, R. Rickert, E. J. Barzilay, and J. M. Whichard
Identification of the Aminoglycoside Resistance Determinants armA and rmtC among Non-Typhi Salmonella Isolates from Humans in the United States
Antimicrob. Agents Chemother., October 1, 2009; 53(10): 4563 - 4564.
[Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Q. Wu, Y. Zhang, L. Han, J. Sun, and Y. Ni
Plasmid-Mediated 16S rRNA Methylases in Aminoglycoside-Resistant Enterobacteriaceae Isolates in Shanghai, China
Antimicrob. Agents Chemother., January 1, 2009; 53(1): 271 - 272.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
C. C. Papagiannitsis, L. S. Tzouvelekis, and V. Miriagou
Relative Strengths of the Class 1 Integron Promoter Hybrid 2 and the Combinations of Strong and Hybrid 1 with an Active P2 Promoter
Antimicrob. Agents Chemother., January 1, 2009; 53(1): 277 - 280.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J. M. Adams-Haduch, D. L. Paterson, H. E. Sidjabat, A. W. Pasculle, B. A. Potoski, C. A. Muto, L. H. Harrison, and Y. Doi
Genetic Basis of Multidrug Resistance in Acinetobacter baumannii Clinical Isolates at a Tertiary Medical Center in Pennsylvania
Antimicrob. Agents Chemother., November 1, 2008; 52(11): 3837 - 3843.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
Y. Zhang, H. Zhou, X.-q. Shen, P. Shen, Y.-s. Yu, and L.-j. Li
Plasmid-borne armA methylase gene, together with blaCTX-M-15 and blaTEM-1, in a Klebsiella oxytoca isolate from China
J. Med. Microbiol., October 1, 2008; 57(10): 1273 - 1276.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
T. R. Fritsche, M. Castanheira, G. H. Miller, R. N. Jones, and E. S. Armstrong
Detection of Methyltransferases Conferring High-Level Resistance to Aminoglycosides in Enterobacteriaceae from Europe, North America, and Latin America
Antimicrob. Agents Chemother., May 1, 2008; 52(5): 1843 - 1845.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
H. Y. Kang, K. Y. Kim, J. Kim, J. C. Lee, Y. C. Lee, D. T. Cho, and S. Y. Seol
Distribution of Conjugative-Plasmid-Mediated 16S rRNA Methylase Genes among Amikacin-Resistant Enterobacteriaceae Isolates Collected in 1995 to 1998 and 2001 to 2006 at a University Hospital in South Korea and Identification of Conjugative Plasmids Mediating Dissemination of 16S rRNA Methylase
J. Clin. Microbiol., February 1, 2008; 46(2): 700 - 706.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J.-i. Wachino, K. Shibayama, H. Kurokawa, K. Kimura, K. Yamane, S. Suzuki, N. Shibata, Y. Ike, and Y. Arakawa
Novel Plasmid-Mediated 16S rRNA m1A1408 Methyltransferase, NpmA, Found in a Clinically Isolated Escherichia coli Strain Resistant to Structurally Diverse Aminoglycosides
Antimicrob. Agents Chemother., December 1, 2007; 51(12): 4401 - 4409.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
D. M. Weinthal, I. Barash, M. Panijel, L. Valinsky, V. Gaba, and S. Manulis-Sasson
Distribution and Replication of the Pathogenicity Plasmid pPATH in Diverse Populations of the Gall-Forming Bacterium Pantoea agglomerans
Appl. Envir. Microbiol., December 1, 2007; 73(23): 7552 - 7561.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. Golebiewski, I. Kern-Zdanowicz, M. Zienkiewicz, M. Adamczyk, J. Zylinska, A. Baraniak, M. Gniadkowski, J. Bardowski, and P. Ceglowski
Complete Nucleotide Sequence of the pCTX-M3 Plasmid and Its Involvement in Spread of the Extended-Spectrum {beta}-Lactamase Gene blaCTX-M-3
Antimicrob. Agents Chemother., November 1, 2007; 51(11): 3789 - 3795.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Y. Doi, J. M. Adams, K. Yamane, and D. L. Paterson
Identification of 16S rRNA Methylase-Producing Acinetobacter baumannii Clinical Strains in North America
Antimicrob. Agents Chemother., November 1, 2007; 51(11): 4209 - 4210.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
L. Chen, Z.-L. Chen, J.-H. Liu, Z.-L. Zeng, J.-Y. Ma, and H.-X. Jiang
Emergence of RmtB methylase-producing Escherichia coli and Enterobacter cloacae isolates from pigs in China
J. Antimicrob. Chemother., May 1, 2007; 59(5): 880 - 885.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Y. Doi, D. de Oliveira Garcia, J. Adams, and D. L. Paterson
Coproduction of Novel 16S rRNA Methylase RmtD and Metallo-{beta}-Lactamase SPM-1 in a Panresistant Pseudomonas aeruginosa Isolate from Brazil
Antimicrob. Agents Chemother., March 1, 2007; 51(3): 852 - 856.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J.-i. Wachino, K. Yamane, K. Kimura, N. Shibata, S. Suzuki, Y. Ike, and Y. Arakawa
Mode of Transposition and Expression of 16S rRNA Methyltransferase Gene rmtC Accompanied by ISEcp1.
Antimicrob. Agents Chemother., September 1, 2006; 50(9): 3212 - 3215.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
M. A. Toleman, P. M. Bennett, and T. R. Walsh
ISCR Elements: Novel Gene-Capturing Systems of the 21st Century?
Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 296 - 316.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.