Skip Navigation



JAC Advance Access published online on July 8, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki238
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/3/481    most recent
dki238v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Tristram, S. G.
Right arrow Articles by Souprounov, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tristram, S. G.
Right arrow Articles by Souprounov, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received March 2, 2005
Revised May 24, 2005
Accepted June 8, 2005

Original article

Variation in selected regions of blaTEM genes and promoters in Haemophilus influenzae

Stephen G. Tristram 1*, Rebecca Hawes 1, and Juliana Souprounov 1

1 School of Human Life Sciences, University of Tasmania, Launceston, Tasmania 7250, Australia

* To whom correspondence should be addressed.
Stephen G. Tristram, E-mail: Stephen.Tristram{at}utas.edu.au


  Abstract

Objectives: To determine if the TEM {beta}-lactamases of Haemophilus influenzae are TEM-1 or derivatives thereof and associated exclusively with the overlapping Pa/Pb promoters.

Methods: Single nucleotide specific PCR was used to discriminate the polymorphic nucleotides at positions 32 and 317 of the blaTEM genes of a collection of TEM-positive strains.

Results: All blaTEM genes were found to be blaTEM-1 or derivatives thereof and none blaTEM-2. The blaTEM genes were associated with the P3 promoter, the Pa/Pb promoters or a novel promoter produced as a result of a 135 bp deletion and a G162T substitution.

Conclusions: The genetic features of blaTEM genes in H. influenzae are different from those in Enterobacteriaceae and more variable than previously recognized.

Keywords: H. influenzae; promoters; {beta}-lactamases; blaTEM genes; TEM.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
S. Garcia-Cobos, J. Campos, E. Cercenado, F. Roman, E. Lazaro, M. Perez-Vazquez, F. de Abajo, and J. Oteo
Antibiotic Resistance in Haemophilus influenzae Decreased, except for {beta}-Lactamase-Negative Amoxicillin-Resistant Isolates, in Parallel with Community Antibiotic Consumption in Spain from 1997 to 2007
Antimicrob. Agents Chemother., August 1, 2008; 52(8): 2760 - 2766.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
S. Garcia-Cobos, J. Campos, F. Roman, C. Carrera, M. Perez-Vazquez, B. Aracil, and J. Oteo
Low {beta}-Lactamase-Negative Ampicillin-Resistant Haemophilus influenzae Strains Are Best Detected by Testing Amoxicillin Susceptibility by the Broth Microdilution Method
Antimicrob. Agents Chemother., July 1, 2008; 52(7): 2407 - 2414.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
S. G. Tristram, M. J. Pitout, K. Forward, S. Campbell, S. Nichols, and R. J. Davidson
Characterization of extended-spectrum {beta}-lactamase-producing isolates of Haemophilus parainfluenzae
J. Antimicrob. Chemother., March 1, 2008; 61(3): 509 - 514.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
S. G. Tristram and J. G. Burdach
Effect of cloned inhibitor-resistant TEM {beta}-lactamases on the susceptibility of Haemophilus influenzae to amoxicillin/clavulanate
J. Antimicrob. Chemother., November 1, 2007; 60(5): 1151 - 1154.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
S. Tristram, M. R. Jacobs, and P. C. Appelbaum
Antimicrobial Resistance in Haemophilus influenzae
Clin. Microbiol. Rev., April 1, 2007; 20(2): 368 - 389.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
S. G. Tristram and S. Nichols
A multiplex PCR for {beta}-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant
J. Antimicrob. Chemother., July 1, 2006; 58(1): 183 - 185.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.