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JAC Advance Access published online on June 27, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki236
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received November 9, 2004
Revised May 21, 2005
Accepted June 6, 2005

Brief report

In vitro activity of citropin 1.1 alone and in combination with clinically used antimicrobial agents against Rhodococcus equi

Andrea Giacometti 1*, Oscar Cirioni 2, Wojciech Kamysz 3, Carmela Silvestri 2, Maria Simona Del Prete 2, Alberto Licci 2, Giuseppina D'Amato 2, Jerzy Lukasiak 3, and Giorgio Scalise 2

1 Institute of Infectious Diseases and Public Health, Università Politecnica delle Marche, Ancona, Italy; Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland
2 Institute of Infectious Diseases and Public Health, Università Politecnica delle Marche, Ancona, Italy
3 Faculty of Pharmacy, Medical University of Gdansk, Gdansk, Poland

* To whom correspondence should be addressed.
Andrea Giacometti, E-mail: anconacmi{at}interfree.it


  Abstract

Objectives: The aim of this study was to investigate the in vitro activity of citropin 1.1, an antimicrobial peptide derived from the Australian tree frog Litoria citropa, alone and in combination with ampicillin, ceftriaxone, doxycycline, netilmicin, ciprofloxacin, rifampicin, linezolid, vancomycin, clarithromycin and imipenem against 12 nosocomial isolates of Rhodococcus equi.

Methods: Antimicrobial activity of citropin 1.1 was measured by MIC, MBC, time-kill studies and chequerboard titration method.

Results: All isolates were inhibited at concentrations of citropin 1.1 between 2 and 8 mg/L. Combination studies demonstrated synergy only when the peptide was combined with clarithromycin, doxycycline and rifampicin.

Conclusions: Our findings show that citropin 1.1 is active against R. equi and that its activity could be enhanced when it is combined with hydrophobic antibiotics.

Keywords: Gram-positive cocci; antimicrobial peptides; synergy; antibiotics.
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