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JAC Advance Access published online on June 21, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki203
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received March 16, 2005
Revised May 14, 2005
Accepted May 24, 2005

Original article

Phenotypic detection of {beta}-lactamase-mediated resistance to oxyimino-cephalosporins in Enterobacteriaceae: evaluation of the Mastascan Elite Expert System

T. G. Winstanley 1*, H. K. Parsons 1, M. A. Horstkotte 2, I. Sobottka 2, and E. Stürenburg 2

1 Department of Microbiology, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, UK
2 Institut für Infektionsmedizin, Zentrum für Klinisch-Theoretische Medizin, Universitätsklinikum Hamburg-Eppendorf, Germany

* To whom correspondence should be addressed.
T. G. Winstanley, E-mail: tgwinstanley{at}hotmail.com


  Abstract

Objectives: The aim of this study was to evaluate phenotypic detection of {beta}-lactamase-mediated resistance to oxyimino-cephalosporins in Enterobacteriaceae using the Mastascan Elite Expert System challenged with a battery of genotypically characterized organisms.

Methods: Isolates (n = 120) were identified to species level and antimicrobial susceptibilities were determined using agar incorporation methods and Mastascan Elite. Phenotypes were examined using an Expert System (ES) and putative genotypes were suggested using interpretative reading.

Results: Identification was correct in 119 of 120 isolates. The ES was able to identify the correct {beta}-lactam phenotype (as deduced from molecular methods) in a single choice in 98 of 120 (81.7%) isolates. In an additional 15 (12.5%) cases, the ES identified the correct {beta}-lactam phenotype within two or more choices. The detected phenotype was incorrect in seven (5.8%) isolates, but three of these were not inherent to the ES.

Conclusions: The Mastascan Elite ES is relatively inexpensive and flexible and can identify the mechanism of resistance to oxyimino-cephalosporins in the majority of Enterobacteriaceae without recourse to molecular methods.

Keywords: interpretative reading; expert system; AmpC; CTX-M; K1; ESBLs.
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