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JAC Advance Access published online on May 12, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki160
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received February 1, 2005
Revised April 11, 2005
Accepted April 15, 2005

Original article

Prevalence of Ambler class A and D {beta}-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea

Seungok Lee 1, Yeon-Joon Park 2*, Myungshin Kim 2, Hae Kyung Lee 2, Kyungja Han 2, Chang Suk Kang 2, and Moon Won Kang 3

1 Seoul Medical Science Institute, Seoul Clinical Laboratories, Seoul, Korea
2 Department of Clinical Pathology, College of Medicine, The Catholic University of Korea, Kangnam St Mary's Hospital, 505 Banpo-dong, Seocho-ku, Seoul, 137-701, Korea
3 Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea

* To whom correspondence should be addressed.
Yeon-Joon Park, E-mail: yjpk{at}catholic.ac.kr


  Abstract

Objectives: Recently, resistance to extended-spectrum cephalosporins due to acquired {beta}-lactamases has been reported in Pseudomonas aeruginosa. The aim of this study was to investigate the prevalence of Ambler class A and D {beta}-lactamases and their extended-spectrum derivatives and antimicrobial susceptibilities of P. aeruginosa isolated from various parts of Korea.

Methods: A total of 252 consecutive, non-duplicate isolates of P. aeruginosa were studied for the presence of class A or D {beta}-lactamase. Antibiotic susceptibility tests and PCR amplification of genes encoding class A (blaPSE-1, blaPER-1, blaVEB-1, blaTEM, blaSHV, blaCTX-M and blaGES-1) and class D {beta}-lactamases (blaOXA-groupI, blaOXA-groupII and blaOXA-groupIII) were performed. For PCR-positive isolates, isoelectric focusing (IEF) analysis, sequencing and pulsed-field gel electrophoresis (PFGE) were performed.

Results: In 64 (25.4%) isolates, structural genes for PSE-1 (6.3%), OXA-10 (13.1%), OXA-4 (4.3%), OXA-30 (2.0%), OXA-2 (2.3%) and OXA-17 (0.4%) were found; their distribution varied between provinces. None harboured blaPER-1, blaVEB-1, blaTEM, blaSHV, blaCTX-M and blaGES-1. The cross-class resistance rates to other antibiotics was significantly higher in class A and D {beta}-lactamase producers than in non-producers (P < 0.001 for aminoglycosides, ciprofloxacin and meropenem).

Conclusions: OXA-type {beta}-lactamases are widespread, but their extended-spectrum derivatives are rare among P. aeruginosa in Korea. To our knowledge, this is the first report of OXA-17, an extended-spectrum derivative of OXA-10, outside the Middle East. In addition, combined resistance to ticarcillin and aminoglycosides was a useful indicator for P. aeruginosa producing PSE- or OXA-type {beta}-lactamases in this study.

Keywords: P. aeruginosa; ESBLs; extended-spectrum {beta}-lactamases; OXA-17.
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