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JAC Advance Access published online on May 4, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki118
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received November 16, 2004
Revised March 10, 2005
Accepted March 11, 2005

Original article

Genetic analysis of pbp2x in clinical Streptococcus pneumoniae isolates in Quebec, Canada

Dominic Granger 1, Geneviève Boily-Larouche 2, Pierre Turgeon 3, Karl Weiss 4, and Michel Roger 1*

1 Laboratoire d'immunogénétique, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CHUM), Montréal, Canada; Département de microbiologie-immunologie, Hôpital Notre-Dame du CHUM, 1560 Sherbrooke Est, Montréal, Québec, Canada H2L 4M1
2 Laboratoire d'immunogénétique, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CHUM), Montréal, Canada
3 Département de microbiologie-immunologie, Hôpital Notre-Dame du CHUM, 1560 Sherbrooke Est, Montréal, Québec, Canada H2L 4M1; Département de microbiologie, Hôpital Saint-Luc du CHUM, Montréal, Canada
4 Département de microbiologie-immunologie, Hôpital Notre-Dame du CHUM, 1560 Sherbrooke Est, Montréal, Québec, Canada H2L 4M1; Département de microbiologie, Hôpital Maisonneuve-Rosemont, Montréal, Canada

* To whom correspondence should be addressed.
Michel Roger, E-mail: michel.roger{at}ssss.gouv.qc.ca


  Abstract

Objectives: To investigate the nature of the amino acid motifs found in penicillin-binding protein (PBP) 2x of penicillin-resistant Streptococcus pneumoniae isolates across the province of Quebec (Canada), and to obtain preliminary information regarding the prevalence of these alterations.

Methods: The pbp2x genomic region encompassing codons 178-703, which includes the entire region of the transpeptidase domain, was sequenced and compared for 52 clinical isolates comprising 20 penicillin-susceptible (PSSP), 20 penicillin-intermediate (PISP) and 12 penicillin-resistant (PRSP) pneumococci.

Results: The degree of diversity within PBP2x correlated with increased resistance to {beta}-lactam antibiotics. There were an average of 5.0 ± 1.8 mutations in PSSP, 37.9 ± 4.4 in PISP, and 63.0 ± 2.0 in PRSP isolates when compared with the control penicillin-susceptible strain R6. At least six distinct amino acid profiles were identified among PISP strains isolated in Quebec. In contrast, all PRSP isolates shared a similar pattern of altered amino acids compared with the sequence from susceptible strains.

Conclusions: These data will be useful in future studies to monitor the genetic changes associated with the emergence and spread of {beta}-lactam resistance in Quebec.

Keywords: penicillin-binding proteins; {beta}-lactams; pneumococci; serotype; penicillin resistance; cefotaxime resistance.
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