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JAC Advance Access published online on March 10, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki081
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JAC © The British Society for Antimicrobial Chemotherapy 2005; all rights reserved
Received January 5, 2005
Revised January 31, 2005
Accepted February 2, 2005

Brief report

Class 1 integron-associated gene cassettes in Salmonella enterica subsp. enterica serovar Agona isolated from pig carcasses in Brazil

Geovana Brenner Michael 1, Marisa Cardoso 2, and Stefan Schwarz 3*

1 Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft (FAL), Höltystr. 10, 31535 Neustadt-Mariensee, Germany; Departamento de Medicina Veterinária Preventiva, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil
2 Departamento de Medicina Veterinária Preventiva, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil
3 Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft (FAL), Höltystr. 10, 31535 Neustadt-Mariensee, Germany

* To whom correspondence should be addressed.
Stefan Schwarz, E-mail: stefan.schwarz{at}fal.de


  Abstract

Objectives: Two multiresistant Salmonella enterica subsp. enterica serovar Agona isolates from pig carcasses were investigated for antimicrobial resistance genes and their location with particular reference to the detection of class 1 integrons.

Methods: The two S. Agona isolates were investigated for their in vitro susceptibility to antimicrobial agents and their plasmid content. The resistance genes and class 1 amplicons were identified by PCR assays. Amplicons of class 1 integrons were cloned and sequenced. Transferability of resistance plasmids was confirmed by conjugation.

Results: Both S. Agona isolates carried conjugative plasmids of approximately 150 kb which harboured all resistance genes detected in the respective isolates. S. Agona 231 was resistant to chloramphenicol by catA1, to tetracycline and minocycline by tet(B), and to sulphonamides by sul1. In addition, it harboured a streptomycin resistance gene strA and a class 1 integron with a new aadA variant designated aadA23, which mediates resistance to streptomycin and spectinomycin. S. Agona 242 also carried the genes catA1, tet(B), and sul1. Moreover, it harboured a second sulphonamide resistance gene, sul2, and a class 1 integron with intact gene cassettes carrying new variants of the trimethoprim resistance gene dfrA15b or the chloramphenicol resistance gene cmlA4. The third gene cassette consisted of a truncated aadA2 gene.

Conclusions: The results of this study show that large conjugative multiresistance plasmids are present in S. Agona from pigs. Analysis of the class 1 integrons revealed the presence of new variants of resistance genes so far not detected in Salmonella isolates.

Keywords: 59-base element; trimethoprim; chloramphenicol; streptomycin; spectinomycin; resistance.
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