JAC Advance Access published online on March 10, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki076
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Microbiology, Kyungpook National University School of Medicine, 101 Dongin-dong, Jung-gu, Daegu 700-422, Korea
* To whom correspondence should be addressed. Objectives: Antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea were characterized. Methods: E. coli isolates were examined for susceptibility to antimicrobial agents. Integrase genes were amplified. Gene cassette regions for classes 1 and 2 integrons were amplified and sequenced. Conjugal transfer and Southern hybridization were performed to determine the genetic localization of class 1 integrons. The clonal relationship of E. coli isolates carrying an identical cassette array was analysed by PFGE. Results: Commensal E. coli isolates from animals were highly resistant to commonly used antimicrobial agents such as tetracycline, sulfamethoxazole, streptomycin, ampicillin and carbenicillin. Integrons were most prevalent in commensal E. coli isolates from poultry (44%), followed by clinical isolates from humans (33%), commensal isolates from swine (23%) and humans (13%). dfrA17-aadA5, dfrA12-orfF-aadA2 and aadA1 were found most frequently in E. coli isolates from humans, poultry and swine, respectively. Class 1 integrons were mostly located in conjugative plasmids. E. coli isolates carrying an identical cassette array were phylogenetically unrelated. Conclusions: The use of antibiotics is strongly associated with antimicrobial resistance. E. coli isolates from different sources may select a specific gene cassette by antibiotic selective pressure, which results in differences in class 1 integrons. The horizontal transfer of class 1 integrons through conjugative plasmids seems to be responsible for wide dissemination of a particular type of class 1 integron.
Received December 15, 2004
Revised January 21, 2005
Accepted January 26, 2005
Original article
Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea
2 Institute of Infectious Diseases, Kyungpook National University School of Medicine, 101 Dongin-dong, Jung-gu, Daegu700-422, Korea and Department of Clinical Pathology, Kyungpook National University School of Medicine, 101 Dongin-dong, Jung-gu, Daegu 700-422, Korea
3 Department of Microbiology, Kyungpook National University School of Medicine, 101 Dongin-dong, Jung-gu, Daegu 700-422, Korea, Institute of Infectious Diseases, Kyungpook National University School of Medicine, 101 Dongin-dong, Jung-gu, Daegu700-422, Korea and
4 Department of Microbiology, Kyungpook National University School of Medicine, 101 Dongin-dong, Jung-gu, Daegu 700-422, Korea, Institute of Infectious Diseases, Kyungpook National University School of Medicine, 101 Dongin-dong, Jung-gu, Daegu700-422, Korea and
Je Chul Lee, E-mail: leejc{at}knu.ac.kr
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. T. Lim, C. C. Yeo, R. Md Yasin, G. Balan, and K. L. Thong Characterization of multidrug-resistant and extended-spectrum {beta}-lactamase-producing Klebsiella pneumoniae strains from Malaysian hospitals J. Med. Microbiol., November 1, 2009; 58(11): 1463 - 1469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Rayamajhi, S. B. Cha, M. L. Kang, S. I. Lee, H. S. Lee, and H. S. Yoo Inter- and Intraspecies Plasmid-Mediated Transfer of Florfenicol Resistance in Enterobacteriaceae Isolates from Swine Appl. Envir. Microbiol., September 1, 2009; 75(17): 5700 - 5703. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Radhouani, P. Poeta, G. Igrejas, A. Goncalves, L. Vinue, and C. Torres Antimicrobial resistance and phylogenetic groups in isolates of Escherichia coli from seagulls at the Berlengas nature reserve Vet Rec., August 1, 2009; 165(5): 138 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Koljalg, K. Truusalu, I. Vainumae, J. Stsepetova, E. Sepp, and M. Mikelsaar Persistence of Escherichia coli Clones and Phenotypic and Genotypic Antibiotic Resistance in Recurrent Urinary Tract Infections in Childhood J. Clin. Microbiol., January 1, 2009; 47(1): 99 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Vinue, Y. Saenz, S. Somalo, E. Escudero, M. A. Moreno, F. Ruiz-Larrea, and C. Torres Prevalence and diversity of integrons and associated resistance genes in faecal Escherichia coli isolates of healthy humans in Spain J. Antimicrob. Chemother., November 1, 2008; 62(5): 934 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kadlec and S. Schwarz Analysis and distribution of class 1 and class 2 integrons and associated gene cassettes among Escherichia coli isolates from swine, horses, cats and dogs collected in the BfT-GermVet monitoring study J. Antimicrob. Chemother., September 1, 2008; 62(3): 469 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Machado, T. M. Coque, R. Canton, J. C. Sousa, and L. Peixe Antibiotic resistance integrons and extended-spectrum {beta}-lactamases among Enterobacteriaceae isolates recovered from chickens and swine in Portugal J. Antimicrob. Chemother., August 1, 2008; 62(2): 296 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-E. Kim, Y.-W. Jeong, S.-H. Cho, S.-J. Kim, and H.-J. Kwon Chronological Study of Antibiotic Resistances and Their Relevant Genes in Korean Avian Pathogenic Escherichia coli Isolates J. Clin. Microbiol., October 1, 2007; 45(10): 3309 - 3315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Daikos, C. Kosmidis, P. T. Tassios, G. Petrikkos, A. Vasilakopoulou, M. Psychogiou, I. Stefanou, A. Avlami, and N. Katsilambros Enterobacteriaceae Bloodstream Infections: Presence of Integrons, Risk Factors, and Outcome Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2366 - 2372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Frank, V. Gautier, A. Talarmin, R. Bercion, and G. Arlet Characterization of sulphonamide resistance genes and class 1 integron gene cassettes in Enterobacteriaceae, Central African Republic (CAR) J. Antimicrob. Chemother., April 1, 2007; 59(4): 742 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pallecchi, C. Lucchetti, A. Bartoloni, F. Bartalesi, A. Mantella, H. Gamboa, A. Carattoli, F. Paradisi, and G. M. Rossolini Population Structure and Resistance Genes in Antibiotic-Resistant Bacteria from a Remote Community with Minimal Antibiotic Exposure Antimicrob. Agents Chemother., April 1, 2007; 51(4): 1179 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sunde and M. Norstrom The prevalence of, associations between and conjugal transfer of antibiotic resistance genes in Escherichia coli isolated from Norwegian meat and meat products J. Antimicrob. Chemother., October 1, 2006; 58(4): 741 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bertrand, F.-X. Weill, A. Cloeckaert, M. Vrints, E. Mairiaux, K. Praud, K. Dierick, C. Wildemauve, C. Godard, P. Butaye, et al. Clonal Emergence of Extended-Spectrum {beta}-Lactamase (CTX-M-2)-Producing Salmonella enterica Serovar Virchow Isolates with Reduced Susceptibilities to Ciprofloxacin among Poultry and Humans in Belgium and France (2000 to 2003). J. Clin. Microbiol., August 1, 2006; 44(8): 2897 - 2903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. W. Stokes, C. L. Nesbo, M. Holley, M. I. Bahl, M. R. Gillings, and Y. Boucher Class 1 integrons potentially predating the association with tn402-like transposition genes are present in a sediment microbial community. J. Bacteriol., August 1, 2006; 188(16): 5722 - 5730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Skurnik, R. Ruimy, A. Andremont, C. Amorin, P. Rouquet, B. Picard, and E. Denamur Effect of human vicinity on antimicrobial resistance and integrons in animal faecal Escherichia coli J. Antimicrob. Chemother., June 1, 2006; 57(6): 1215 - 1219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. E. Sidjabat, K. M. Townsend, N. D. Hanson, J. M. Bell, H. W. Stokes, K. S. Gobius, S. M. Moss, and D. J. Trott Identification of blaCMY-7 and associated plasmid-mediated resistance genes in multidrug-resistant Escherichia coli isolated from dogs at a veterinary teaching hospital in Australia J. Antimicrob. Chemother., May 1, 2006; 57(5): 840 - 848. [Abstract] [Full Text] [PDF]
|
|