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JAC Advance Access published online on March 16, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dki065
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received November 3, 2004
Revised January 3, 2005
Accepted January 18, 2005

Original article

Genetic and biochemical characterization of the chromosome-encoded class B {beta}-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1)

Laurent Poirel 1, Claire Héritier 1, and Patrice Nordmann 1*

1 Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France

* To whom correspondence should be addressed.
Patrice Nordmann, E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr


  Abstract

Objectives: To determine the {beta}-lactamase gene content of {beta}-lactam-susceptible psychrophilic Gram-negative bacilli, Shewanella frigidimarina and Shewanella livingstonensis, isolated from a marine environment.

Methods: {beta}-Lactamase genes were cloned, sequenced and expressed in Escherichia coli. Kinetic parameters were determined using purified enzymes.

Results: Metallo-{beta}-lactamases SLB-1 and SFB-1 were identified from S. livingstonensis and S. frigidimarina, respectively, sharing 65% amino acid identity and being distantly related to other Ambler class B {beta}-lactamases (40 and 36% amino acid identity with GIM-1 and IMP-1 from Pseudomonas aeruginosa, respectively). SLB-1 had an EDTA-inhibited and broad-spectrum {beta}-lactam hydrolysis profile, whereas SFB-1 did not hydrolyse cephalosporins, with activity being weakly inhibited by EDTA and dipicolinic acid.

Conclusions: This work provides further evidence that psychrophilic bacterial species may constitute a reservoir of {beta}-lactam resistance genes.

Keywords: metallo-{beta}-lactamases; S. livingstonensis; S. frigidimarina; carbapenems.
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