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JAC Advance Access published online on March 2, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh556
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JAC © The British Society for Antimicrobial Chemotherapy 2005; all rights reserved
Received September 16, 2004
Revised November 11, 2004
Accepted November 27, 2004

Original article

Description of In116, the first blaCTX-M-2-containing complex class 1 integron found in Morganella morganii isolates from Buenos Aires, Argentina

Pablo Power 1, Moreno Galleni 2, José Di Conza 1, Juan A. Ayala 3, and Gabriel Gutkind 1*

1 Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 (1113), Buenos Aires, Argentina
2 Centre d'Ingéniérie des Protéines, Université de Liège, Institut de Chimie, B6 (B-4000), Sart Tilman, Belgium
3 Centro de Biología Molecular ‘Severo Ochoa’, CSIC-UAM, Campus de Cantoblanco (28049), Madrid, Spain

* To whom correspondence should be addressed.
Gabriel Gutkind, E-mail: ggutkind{at}ffyb.uba.ar


  Abstract

Objectives: We analysed the architecture and probable origin of a class 1 integron from cefotaxime-resistant Morganella morganii isolates.

Methods: bla genes and class 1 integron elements were detected by PCR and DNA-DNA hybridization in a M. morganii strain isolated in 1996. PCR-mapping and sequencing of different fragments were carried out to determine the integron's architecture.

Results and conclusions: A class 1 integron (In116), strongly related to the In6/In7 family, was detected in a plasmid from an oxyimino-cephalosporin-resistant M. morganii strain, producing CTX-M-2 {beta}-lactamase. The variable region of In116 contains aacA4, blaOXA-2 and orfD cassettes. Downstream of the 3'-conserved-segment (3'-CS), an orf513-containing common region is followed by blaCTX-M-2 and flanking regions, having 96-99% nucleotide identity with Kluyvera ascorbata's kluA-1 and neighbouring sequences. Some of the evidence supporting the incorporation of foreign DNA is as follows: a partial deletion in a second 3'-CS (3'-CS2), and the absence of 59-base element or IS-like structures upstream of blaCTX-M-2.

Keywords: Kluyvera ascorbata; In6/In7; orf513; aacA4; blaOXA-2.
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