JAC Advance Access published online on January 28, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh543
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1 Department
of Veterinary Microbiology & Parasitology, Faculty of Veterinary Medicine,
University College Dublin, Belfield, Dublin 4, Ireland
and
* To whom correspondence should be addressed. Objectives: A complete gene cassette
contained in a class 1 integron from a multidrug-resistant (MDR) isolate
of Acinetobacter baumannii cultured from a horse was characterized by
molecular methods. Methods: Template genomic DNA purified from
the A. baumannii isolate was investigated by PCR. A gene
cassette-associated amplicon was detected and completely
characterized. Results: A 2.6 kbp DNA fragment
containing four gene cassettes was amplified from the MDR A. baumannii
isolate. Sequence analysis showed it was similar to sequences recently reported
in Klebsiella pneumoniae, Serratia marcescens and an
Escherichia coli plasmid p1658/97 which conferred aminoglycoside
resistance. Aminoglycoside resistance-encoding genes aacC1 and
aadA1 were located within the 2.6 kbp amplicon, separated by
two open reading frames (ORFs) coding for unknown products. This cassette
structure (and some variants) was identified in unrelated
Acinetobacter spp. from human sources, based on sequence comparisons
of the current databases. Conclusions: Identification of a
complete class 1 integron in an equine isolate of A. baumannii
suggests that the screening of isolates from animals for these elements should
be considered, as this information could influence the selection of
chemotherapeutic agents.
Received September 3, 2004
Revised November 16, 2004
Accepted November 19, 2004
Brief report
Characterization of a 2.6 kbp
variable region within a class 1 integron found in an Acinetobacter
baumannii strain isolated from a horse
,,
2 Centre
for Food Safety, Faculty of Veterinary Medicine, University College Dublin,
Belfield, Dublin 4, Ireland
3 Department
of Veterinary Microbiology & Parasitology, Faculty of Veterinary Medicine,
University College Dublin, Belfield, Dublin 4, Ireland
4 Department of
Veterinary Microbiology & Parasitology, Faculty of Veterinary Medicine,
University College Dublin, Belfield, Dublin 4, Ireland
5 Department of Infectious Diseases C5-P,
Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The
Netherlands
6 Department
of Infectious Diseases C5-P, Leiden University Medical Center, P.O. Box
9600, 2300 RC Leiden, The Netherlands
Séamus Fanning, E-mail: sfanning{at}ucd.ie
Abstract The
first two authors contributed equally to this study.
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