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JAC Advance Access published online on January 13, 2005
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh541
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JAC © The British Society for Antimicrobial Chemotherapy 2005; all rights reserved
Received July 26, 2004
Revised November 18, 2004
Accepted November 19, 2004

Brief report

Enhanced resistance to erythromycin is conferred by the enterococcal msrC determinant in Staphylococcus aureus

Elinor Reynolds 1 and Jonathan H. Cove 1*

1 School of Biochemistry and Microbiology, University of Leeds, Leeds LS2 9JT, UK

* To whom correspondence should be addressed.
Jonathan H. Cove, E-mail: j.h.cove{at}leeds.ac.uk


  Abstract

The msrC gene, found on the chromosome of Enterococcus faecium, shares a high degree of similarity with the staphylococcal erythromycin resistance determinant msr(A). The enterococcal determinant was cloned into Staphylococcus aureus to determine whether msrC could confer antibiotic resistance in staphylococci.

A shuttle vector comprising pBluescript and pSK265 was used to introduce multiple copies of msrC into S. aureus RN4220. The integration vector pCL84 was employed to insert a single copy of msrC into the S. aureus chromosome. MICs were determined by the broth microdilution method.

Expression of msrC from both chromosomal and plasmid loci in erythromycin-susceptible S. aureus RN4220 (MIC 0.25 mg/L) gave rise to enhanced protection against erythromycin, with an MIC of 32-64 mg/L for S. aureus RN4220 containing msrC in multiple copies and an MIC of 16-64 mg/L with msrC inserted as a single copy in the S. aureus chromosome.

MsrC mediates high-level resistance to erythromycin in S. aureus.

Keywords: macrolide resistance; ABC transporter; molecular cloning; staphylococci; enterococci.
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