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JAC Advance Access published online on November 16, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh486
© 2004 by The British Society for Antimicrobial Chemotherapy
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Received April 27, 2004
Revised August 11, 2004
Accepted September 29, 2004

Brief report

Nosocomial outbreak by Proteus mirabilis producing extended-spectrum {beta}-lactamase VEB-1 in a Korean university hospital

Ja-Young Kim 1, Yeon-Joon Park 1*, Sang-Il Kim 2, Moon Won Kang 2, Seung-Ok Lee 1, and Kyo-Young Lee 1

1 Department of Clinical Pathology, College of Medicine, The Catholic University of Korea, Kangnam St Mary's Hospital, 505 Banpo-dong, Seocho-ku, Seoul 137-701, Korea
2 Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Kangnam St Mary's Hospital, 505 Banpo-dong, Seocho-ku, Seoul, 137-701, Korea

* To whom correspondence should be addressed.
Yeon-Joon Park, E-mail: yjpk{at}catholic.ac.kr


  Abstract

Objectives: To examine the molecular mechanisms involved in the {beta}-lactam resistance of multidrug-resistant Proteus mirabilis isolates that showed an unusual synergy between imipenem and ceftazidime in a Korean hospital.

Methods: Over an 11 month period, a total of 12 P. mirabilis isolates showing resistance to ampicillin, gentamicin, ceftazidime, cefotaxime, cefuroxime, cefalothin, cefepime, piperacillin, trimethoprim/sulfamethoxazole and ciprofloxacin, were recovered from the sputum and urine specimens of nine patients who were hospitalized in the neurosurgery ward. The extended-spectrum {beta}-lactamases were screened with a double disc synergy test using ceftazidime, cefotaxime, aztreonam, cefepime and clavulanate. The ESBL types were determined by PCR using specific primers for blaTEM-1, blaSHV-1, blaCTX-M-1, blaCTX-M-2, blaCTX-M-8, blaCTX-M-9, blaPER-1, blaGES-1, blaVEB-1, blaOXA-10 and blaOXA-13 followed by sequencing. All the isolates underwent molecular typing by PFGE. The transferability was examined by conjugation.

Results and conclusions: All the isolates showed a marked synergy between the extended-spectrum cephalosporins and clavulanate together with an unusual synergy between cefoxitin and the cephalosporins (cefalothin, cefuroxime, ceftazidime, cefotaxime) and between imipenem, and ceftazidime and cefotaxime. Isoelectric focusing of the crude bacterial extracts showed a {beta}-lactamase band with a pI value of 5.4, which was inhibited by clavulanate. PCR and sequencing identified the gene to be blaVEB-1. In addition, the aadB gene was detected, conferring aminoglycoside resistance. The resistance was not transferred by conjugation. The outbreak was of a clonal origin as shown by PFGE demonstrating an identical banding pattern. This is the first report of VEB-1-producing Enterobacteriaceae in Korea.

Keywords: P. mirabilis; ESBLs; {beta}-lactam resistance; multidrug-resistant.
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