Skip Navigation



JAC Advance Access published online on October 7, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh459
© 2004 by The British Society for Antimicrobial Chemotherapy
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/5/940    most recent
dkh459v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Chryssanthou, E.
Right arrow Articles by Sjölin, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chryssanthou, E.
Right arrow Articles by Sjölin, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received June 3, 2004
Revised August 30, 2004
Accepted September 12, 2004

Brief report

Post-antifungal effect of amphotericin B and voriconazole against Aspergillus fumigatus analysed by an automated method based on fungal CO2 production: dependence on exposure time and drug concentration

Erja Chryssanthou 1* and Jan Sjölin 2

1 Department of Clinical Microbiology L202, Karolinska University Hospital, S-171 76 Stockholm, Sweden
2 Section of Infectious Diseases, Department of Medical Sciences, University Hospital, Uppsala, Sweden

* To whom correspondence should be addressed. E-mail: Erja.Chryssanthou{at}kus.se.


  Abstract

The post-antifungal effect (PAFE) of amphotericin B and voriconazole, either alone or in combination, on Aspergillus fumigatus was studied using an automated system based on fungal CO2 production.

Methods: Conidia of A. fumigatus were exposed to concentrations of 1-10 x MIC of amphotericin B and 1-40 x MIC of voriconazole for 1, 2 and 4 h. After a washing step, exposed and control conidia were inoculated into Pedi-BacT culture bottles. CO2 production was automatically monitored until the bottles signalled positive. The difference in time span for positive signals in drug-exposed and control bottles was used to calculate PAFE.

Results: There was a linear relationship between inoculum size and time to positive signal (r2=0.99). The precision of duplicate analyses was 1.5%. Longer exposure times increased the amphotericin B-induced PAFE (P<0.001), whereas concentrations above the MIC did not. Voriconazole after 4 h of exposure induced a short dose-independent PAFE. The combination with amphotericin B did not prolong the PAFE over that caused by amphotericin B alone.

Conclusions: This automated method can be used for determination of PAFE. In contrast to Candida spp., in which amphotericin B-induced PAFE is mainly related to the area under the curve, the effect on A. fumigatus was more dependent on the exposure time. This implies that pharmacodynamic data obtained from Candida experiments cannot be directly extrapolated to Aspergillus.

Keywords: azoles; antifungals; PAFE; polyenes; susceptibility testing.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
E. Chryssanthou, A. Loebig, and J. Sjolin
Post-antifungal effect of amphotericin B and voriconazole against germinated Aspergillus fumigatus conidia
J. Antimicrob. Chemother., June 1, 2008; 61(6): 1309 - 1311.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.