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JAC Advance Access published online on October 7, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh457
© 2004 by The British Society for Antimicrobial Chemotherapy
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Received July 16, 2004
Revised September 7, 2004
Accepted September 12, 2004

Brief report

Nucleotide sequence and organization of the multiresistance plasmid pSCFS1 from Staphylococcus sciuri

Corinna Kehrenberg 1, Kayode K. Ojo 2, and Stefan Schwarz 1*

1 Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft (FAL), Höltystrasse 10, 31535 Neustadt-Mariensee, Germany
2 Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft (FAL), Höltystrasse 10, 31535 Neustadt-Mariensee, Germany; Department of Pathobiology, University of Washington, Seattle, WA 98195, USA

* To whom correspondence should be addressed. E-mail: stefan.schwarz{at}fal.de.


  Abstract

Objectives: The multiresistance plasmid pSCFS1 from Staphylococcus sciuri was sequenced completely and analysed with regard to its gene organization and the putative role of a novel ABC transporter in antimicrobial resistance.

Methods: Plasmid pSCFS1 was transformed into Staphylococcus aureus RN4220, overlapping restriction fragments were cloned into Escherichia coli plasmid vectors and sequenced. For further analysis of the ABC transporter, a ~3 kb EcoRV-HpaI fragment was cloned into the staphylococcal plasmid pT181MCS and the respective S. aureus RN4220 transformants were subjected to MIC determination.

Results: A total of 14 ORFs coding for proteins of >100 amino acids were detected within the 17 108 bp sequence of pSCFS1. Five of them showed similarity to recombination/mobilization genes while another two were similar to plasmid replication genes. In addition to the previously described genes cfr for chloramphenicol/florfenicol resistance and erm(33) for inducible resistance to macrolide-lincosamide-streptogramin B resistance, a Tn554-like spectinomycin resistance gene and Tn554-related transposase genes were identified. Moreover, a novel ABC transporter was detected and shown to mediate low-level lincosamide resistance.

Conclusion: Plasmid pSCFS1 is composed of various parts which show similarity to sequences known to occur on plasmids or transposons of Gram-positive, but also Gram-negative bacteria. It is likely that pSCFS1 represents the result of inter-plasmid recombination events also involving the truncation of a Tn554-like transposon.

Keywords: macrolide resistance; ABC transporter; low-level clindamycin resistance; florfenicol resistance; spectinomycin resistance.
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