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JAC Advance Access published online on October 7, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh446
© 2004 by The British Society for Antimicrobial Chemotherapy
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Received August 17, 2004
Revised September 1, 2004
Accepted September 3, 2004

Brief report

Identification of a chromosome-borne class C {beta}-lactamase from Erwinia rhapontici

Thierry Naas 1*, Daniel Aubert 1, Sophie Vimont 1, and Patrice Nordmann 1

1 Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France

* To whom correspondence should be addressed. E-mail: thierry.naas{at}bct.ap-hop-paris.fr.


  Abstract

Objectives: To characterize the {beta}-lactamase gene content of Erwinia rhapontici.

Methods: The {beta}-lactamase gene was cloned, sequenced and expressed in Escherichia coli.

Results: The cloned gene conferred a resistance pattern of an Ambler class C {beta}-lactamase in E. coli. The AmpC-type enzyme had a pI value of 8.6 and shared 62% amino acid sequence identity with that of Escherichia fergusonii. The ampC gene was associated with a regulatory ampR gene and {beta}-lactamase production was inducible.

Conclusions: This work provides further evidence of the molecular heterogeneity of {beta}-lactamases in Erwinia spp. and that plant-pathogenic enterobacterial species may constitute a reservoir of antibiotic resistance genes.

Keywords: AmpC; inducible; cephalosporinases; E. rhapontici.
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