JAC Advance Access published online on July 28, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh395
© 2004 by The British Society for Antimicrobial Chemotherapy
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1 Centre Hospitalier Universitaire de Clermont-Ferrand, rue Montalembert, 63000 Clermont-Ferrand, France
* To whom correspondence should be addressed. E-mail: cdechamps{at}chu-reims.fr.
Objectives: To evaluate the frequency and diversity of extended-spectrum Methods: During 2001-2002, all the non-duplicate isolates of P. aeruginosa resistant to ceftazidime and of Enterobacteriaceae intermediate or resistant to ceftazidime and/or cefotaxime and/or aminoglycosides with an AAC(6') I phenotype were collected in nine hospitals of the area. ESBL isoelectric points were determined, bla genes were amplified and sequenced and epidemic isolates were genotyped with ERIC2-PCR. Results: ESBLs were observed in 297 Enterobacteriaceae (0.8%). The most frequent were TEM-3 like (n=152; 51.2%) and TEM-24 (n=115; 38.7%). Four new enzymes were observed, TEM-112 (pI 5.4), TEM-113 (pI 6.3), TEM-114 (pI 5.9) and TEM-126 (pI 5.4). Other TEMs were TEM-8, TEM-12, TEM-16, TEM-19, TEM-20, TEM-21, TEM-29 and TEM-71. The other ESBLs were SHV-4, SHV-5 and SHV-12, CTX-M-1, CTX-M-3, CTX-M-14 and CTX-M-15. In 37 P. aeruginosa (0.7%) only one ESBL was observed, PER-1. Five epidemic strains were detected, Serratia marcescens TEM-3 and four observed in several hospitals, Enterobacter aerogenes TEM-24, Citrobacter koseri TEM-3, Proteus mirabilis TEM-3 and P. aeruginosa PER-1. Conclusion: ESBL frequency was lower than in 1998, and CTX-M-type frequency higher (2.1% of ESBLs in 2001, 4.9% in 2002). This long-term survey detected new sporadic enzymes (TEM-112, TEM-113, TEM-114 and TEM-126) and interhospital epidemic strains while avoiding any overestimation of ESBL frequency that may otherwise have occurred because of acute epidemics.
Revised July 3, 2004
Accepted July 6, 2004
Original article
Frequency and diversity of Class A extended-spectrum
-lactamases in hospitals of the Auvergne, France: a 2 year prospective study
2 Centre Hospitalier de Vichy, Boulevard Denière, 03209 Vichy, France
3 Centre Hospitalier de Roanne, rue de Charlieu, 42328 Roanne, France
4 Centre Hospitalier de Thiers, Chemin Fau, 63300 Thiers, France
5 Centre Hospitalier de Moulins, Avenue du Général de Gaulle, 03009 Moulins, France
6 Centre Hospitalier de Montluçon, Avenue du 8 mai 1945, 03109 Montluçon, France
7 Centre Hospitalier du Puy, Route Montredon, 43000 Le Puy, France
8 Centre Hospitalier de Riom, Boulevard Etienne-Clémentel, 63204 Riom, France
9 Centre Hospitalier d'Aurillac, Avenue de la République, 15000 Aurillac, France
Abstract 
-lactamases (ESBLs) produced by Enterobacteriaceae and Pseudomonas aeruginosa in one French region.
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